公开(公告)号：US06517843B1

公开(公告)日：2003-02-11

申请号：US09583350

申请日：2000-05-31

申请人： John Albert Ellis ,  Gordon Allan Moore ,  Brian Meehan ,  Edward Clark ,  Deborah Haines ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  George Steve Krakowka ,  Jean-Christophe Francis Audonnet ,  Francis McNeilly

发明人： John Albert Ellis ,  Gordon Allan Moore ,  Brian Meehan ,  Edward Clark ,  Deborah Haines ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  George Steve Krakowka ,  Jean-Christophe Francis Audonnet ,  Francis McNeilly

IPC分类号： A61K3912

CPC分类号： C07K14/005 ,  A61K2039/53 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/24043 ,  C12N2750/10022

摘要： Porcine circovirus-2 (PCV-2) is a recently identified agent wherein the potential spectrum of PCV-2-associated disease has been expanded by evidence of vertical and sexual transmission and associated reproductive failure in swine populations. PCV-2 was isolated from a litter of aborted piglets from a farm experiencing late term abortions and stillbirths. Severe, diffuse myocarditis was present in one piglet associated with extensive immunohistochemical staining for PCV-2 antigen. Variable amounts of PCV-2 antigen were also present in liver, lung and kidney of multiple fetuses. Inoculation of female pigs with a composition including an immunogen from PCV-2 or an epitope of interest from such an immunogen or with a vector expressing such an immunogen or epitope of interest prior to breeding, such as within the first five weeks of life, or prior to the perinatal period, or repeatedly over a lifetime, or during pregnancy, such as between the 6th and 8th and/or the 10th and 13th weeks of gestation, can prevent myocarditis, abortion and intrauterine infection associated with porcine circovirus-2. In addition, innoculation of male and/or female pigs with the aforementioned compositions can be carried out to prevent transmission of PCV-2 from male to female (or vice versa) during mating. Thus, the invention involves methods and compositions for preventing myocarditis, abortion and intrauterine infection associated with porcine circovirus-2.

摘要翻译： 猪圆环病毒-2（PCV-2）是最近确定的药物，其中PCV-2相关疾病的潜在谱已经通过垂直和性传播的证据以及猪群体相关的生殖失败而扩大。 从经历了晚期堕胎和死胎的农场的排卵仔猪中分离出PCV-2。 在与PCV-2抗原的广泛免疫组织化学染色相关的一只仔猪中存在严重的弥漫性心肌炎。 不同量的PCV-2抗原也存在于多个胎儿的肝，肺和肾中。 包括来自PCV-2或感兴趣的表位的免疫原的组合物的雌性猪从这样的免疫原或在繁殖之前（例如在生命的前五周内）表达这种免疫原或感兴趣的表位的载体接种，或 在围产期，或一生以上，或怀孕期间，如6〜8和/或10〜13周的妊娠期间，可以预防与猪圆环病毒-2相关的心肌炎，流产和子宫内感染。 此外，可以进行具有上述组成的雄性和/或雌性猪的接种，以防止在交配期间PCV-2从雄性到雌性（或反之亦然）的传播。 因此，本发明涉及用于预防与猪圆环病毒-2相关的心肌炎，流产和子宫内感染的方法和组合物。