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1.发明授权Method of measuring bone resorption activity with a highly enriched population of prefusion osteoclast cells 失效用高度富集的挤压破骨细胞群测量骨吸收活性的方法
公开(公告)号：US6093533A
公开(公告)日：2000-07-25
申请号：US913988
申请日：1997-11-17
CPC分类号： C07K14/745 , C12N5/0643 , G01N33/5008 , G01N33/502 , G01N33/5044 , G01N33/5088 , C12N2502/1311 , C12N2503/02
摘要： Methods of obtaining enriched populations of osteoclast precursor cells which can be released from tissue culture dishes and used for biochemical studies are described. Osteoblastic cells and bone marrow cells are co-cultured. Next a .alpha..sub.v .beta..sub.3 receptor ligand, such as echistatin is used for cell detachment. The result is a 75-95% pure enriched population of tartrate resistant acid phosphatase (TRAP.sup.+) cells, in high yields (2-3.times.10.sup.6 cells per experiment) can be obtained. These cells are mosty mononucleated and based on their characteristics are considered to be pre-fusion osteoclasts (pOC cells). The precursor osteoclasts can be reseeded onto osteoblasts to obtain an enriched population of mature, multinucleated osteoclast cells.
摘要翻译： PCT No.PCT / US96 / 04634。 371日期：1997年11月17日 102（e）1997年11月17日PCT PCT 1996年4月4日PCT公布。 公开号WO96 / 32469 日期1996年10月17日描述了从组织培养皿中释放并用于生物化学研究的获得富含破骨细胞前体细胞群体的方法。 共培养成骨细胞和骨髓细胞。 接下来，使用αvβ3受体配体，例如echistatin用于细胞分离。 结果是可以获得高产率（每个实验2-3×10 6个细胞）的75-95％纯度富含酒石酸盐酸性磷酸酶（TRAP +）细胞的富集群体。 这些细胞是单核细胞，并且基于其特征被认为是预融合的破骨细胞（pOC细胞）。 前体破骨细胞可以重新种植在成骨细胞上，以获得成熟的多核破骨细胞的富集群体。
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公开(公告)号：US5856186A
公开(公告)日：1999-01-05
申请号：US862556
申请日：1997-05-23
IPC分类号： C07K14/745 , C12N5/02 , C12N5/078 , G01N33/50 , C12N5/00
CPC分类号： C07K14/745 , C12N5/0643 , G01N33/5008 , G01N33/502 , G01N33/5044 , G01N33/5088 , C12N2502/1311 , C12N2503/02
摘要： Methods of obtaining enriched populations of osteoclast precursor cells which can be released from tissue culture dishes and used for biochemical studies are described. Osteoblastic cells and bone marrow cells are co-cultured. Next a .alpha..sub.v .beta..sub.3 receptor ligand, such as echistatin is used for cell detachment. The result is an 75-95% pure enriched population of tartrate resistant acid phosphatase (TRAP.sup.+) cells, in high yields (2-3.times.10.sup.6 cells per experiment) can be obtained. These cells are mostly mononucleated and based on their characteristics are considered to be pre-fusion osteoclasts (pOC cells). The precursor osteoclasts can be reseeded onto osteoblasts to obtain an enriched population of mature, multinucleated osteoclast cells.
摘要翻译： 描述了可以从组织培养皿中释放并用于生物化学研究的获得富含破骨细胞前体细胞群体的方法。 共培养成骨细胞和骨髓细胞。 接下来，使用αvβ3受体配体，例如echistatin用于细胞分离。 结果是可以获得高产率（每个实验2-3×10 6个细胞）的75-95％纯度富含酒石酸盐酸性磷酸酶（TRAP +）细胞的富集群体。 这些细胞大多是单核的，并且基于其特征被认为是融合前的破骨细胞（pOC细胞）。 前体破骨细胞可以重新种植在成骨细胞上，以获得成熟的多核破骨细胞的富集群体。
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公开(公告)号：US5719058A
公开(公告)日：1998-02-17
申请号：US419210
申请日：1995-04-10
IPC分类号： C07K14/745 , C12N5/02 , C12N5/078 , G01N33/50 , C12N5/00
CPC分类号： C07K14/745 , C12N5/0643 , G01N33/5008 , G01N33/502 , G01N33/5044 , G01N33/5088 , C12N2502/1311 , C12N2503/02
摘要： Methods of obtaining enriched populations of osteoclast precursor cells which can be released from tissue culture dishes and used for biochemical studies are described. Osteoblastic cells and bone marrow cells are co-cultured. Next a .alpha..sub.v .beta..sub.3 receptor ligand, such as echistatin is used for cell detachment. The result is an 75-95% pure enriched population of tartrate resistant acid phosphatase (TRAP.sup.+) cells, in high yields (2-3.times.10.sup.6 cells per experiment). These cells are mostly mononucleated and based on their characteristics are considered to be pre-fusion osteoclasts (pOC cells). The precursor osteoclasts can be reseeded onto osteoblasts to obtain an enriched population of mature, multinucleated osteoclast cells.
摘要翻译： 描述了可以从组织培养皿中释放并用于生物化学研究的获得富含破骨细胞前体细胞群体的方法。 共培养成骨细胞和骨髓细胞。 接下来，使用αvβ3受体配体，例如echistatin用于细胞分离。 结果是75-95％的富含酒石酸盐酸性磷酸酶（TRAP +）细胞的富集群体，产量高（每个实验2-3×10 6个细胞）。 这些细胞大多是单核的，并且基于其特征被认为是融合前的破骨细胞（pOC细胞）。 前体破骨细胞可以重新种植在成骨细胞上，以获得成熟的多核破骨细胞的富集群体。
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