公开(公告)号：US08907062B2

公开(公告)日：2014-12-09

申请号：US13600963

申请日：2012-08-31

申请人： Hsiu-Chuan Yen ,  Shih-Wei Chen

发明人： Hsiu-Chuan Yen ,  Shih-Wei Chen

IPC分类号： C07K14/00

CPC分类号： C12N9/1007 ,  C12Y201/01201

摘要： The present invention relates to a producing and purifying method of soluble recombinant coenzyme Q5 homolog (COQ5) protein, which is expressed in soluble form by Escherichia coli (E. coli), under native conditions. The method is characterized by pre-treating bacterial lysate with low concentration of ionic detergent, such as sodium dodecyl sulfate (SDS), before purification; and the purifying method is performed under native condition without using urea to avoid the problems of requiring lengthy processes to remove urea in purified protein solution or re-aggregation and precipitation of protein after removal of urea.

摘要翻译： 本发明涉及在天然条件下由大肠杆菌（E.coli）以可溶形式表达的可溶性重组辅酶Q5同源物（COQ5）蛋白的生产和纯化方法。 该方法的特征在于在纯化之前用低浓度的离子洗涤剂如十二烷基硫酸钠（SDS）预处理细菌裂解物; 并且纯化方法在天然条件下进行而不使用尿素，以避免在纯化的蛋白质溶液中需要长时间的方法除去尿素或者在除去尿素后重新聚集和沉淀蛋白质的问题。

公开(公告)号：US20130225792A1

公开(公告)日：2013-08-29

申请号：US13600963

申请日：2012-08-31

申请人： HSIU-CHUAN YEN ,  SHIH-WEI CHEN

发明人： HSIU-CHUAN YEN ,  SHIH-WEI CHEN

IPC分类号： C07K14/00 ,  C12P21/02

CPC分类号： C12N9/1007 ,  C12Y201/01201

摘要： The present invention relates to a producing and purifying method of soluble recombinant COQ5 protein, which is expressed in soluble form by E. coli, under native conditions. The method is characterized by pre-treating bacterial lysate with low concentration of ionic detergent, such as SDS, before purification; and the purifying method is performed under native condition without using urea to avoid the problems of requiring lengthy processes to remove urea in purified protein solution or re-aggregation and precipitation of protein after removal of urea.