公开(公告)号：US10544036B2

公开(公告)日：2020-01-28

申请号：US14888319

申请日：2014-04-30

申请人： Haotian Chen ,  Mark Jeronimo ,  Adrianna McAllister ,  Axel Guenther

发明人： Haotian Chen ,  Mark Jeronimo ,  Adrianna McAllister ,  Axel Guenther

IPC分类号： B81C1/00 ,  B01L3/00

摘要： Methods and devices are disclosed for the controlled formation of structures, including tubular structures, using microfluidic devices. In one embodiment, a microfluidic device includes three or more microfluidic arrays that are provided in a stacking configuration, with an inner streaming layer and an outer streaming layer for flowing a streaming fluid, and at least one intermediate matrix layer for flowing a matrix fluid. Fluid flow in each layer is directed from a peripheral region towards, and distributed around, a common central outlet. Guided by the streaming fluid, a sheath of matrix solution is formed, which may be solidified to form a tubular material. Some embodiments allow for the controlled and continuous extrusion of tubular structures with tailored heterogeneities and/or predictable mechanical and chemical properties. Devices and methods are also provided for the on- and off-chip fixation and optional perfusion of tubular structures.

公开(公告)号：US20160068385A1

公开(公告)日：2016-03-10

申请号：US14888319

申请日：2014-04-30

申请人： Haotian CHEN ,  Mark JERINIMO ,  Arianna MCALLISTER ,  Axel GUENTHER

发明人： Haotian Chen ,  Mark Jeronimo ,  Adrianna McAllister ,  Axel Guenther

IPC分类号： B81C1/00 ,  B01L3/00

CPC分类号： B81C1/00119 ,  B01L3/502707 ,  B01L2200/12 ,  B01L2300/0887 ,  B81B2201/058

摘要： Methods and devices are disclosed for the controlled formation of structures, including tubular structures, using microfluidic devices. In one embodiment, a microfluidic device includes three or more microfluidic arrays that are provided in a stacking configuration, with an inner streaming layer and an outer streaming layer for flowing a streaming fluid, and at least one intermediate matrix layer for flowing a matrix fluid. Fluid flow in each layer is directed from a peripheral region towards, and distributed around, a common central outlet. Guided by the streaming fluid, a sheath of matrix solution is formed, which may be solidified to form a tubular material. Some embodiments allow for the controlled and continuous extrusion of tubular structures with tailored heterogeneities and/or predictable mechanical and chemical properties. Devices and methods are also provided for the on- and off-chip fixation and optional perfusion of tubular structures.

摘要翻译： 公开了用于使用微流体装置控制形成结构（包括管状结构）的方法和装置。 在一个实施例中，微流体装置包括以堆叠配置提供的三个或更多个微流体阵列，其具有用于流动流体流的内部流动层和外部流动层，以及用于流动基质流体的至少一个中间矩阵层。 每个层中的流体流从周边区域引导到共同的中央出口周围并分布在其周围。 由流动液引导，形成基质溶液鞘，其可以固化以形成管状材料。 一些实施例允许具有定制的异质性和/或可预测的机械和化学性质的管状结构的受控和连续挤出。 还提供了用于片外固定和管状结构的可选灌注的装置和方法。