公开(公告)号：US07767409B2

公开(公告)日：2010-08-03

申请号：US11887477

申请日：2005-12-13

申请人： Haruki Mizukami ,  Akira Okuyama ,  Yasuhiko Hatano

发明人： Haruki Mizukami ,  Akira Okuyama ,  Yasuhiko Hatano

IPC分类号： G01N33/53

CPC分类号： G01N33/743 ,  G01N2333/723 ,  G01N2500/04

摘要： Provided is a detection method including exposing, to contact with a surface to which a cofactor has been bound, a nuclear receptor protein serving as a counterpart of the cofactor and a test sample; and detecting a substance which is contained in the test sample and which binds to the nuclear receptor, on the basis of a change in degree of binding between the nuclear receptor protein and the cofactor. The detection method is means for detecting a living-body-related substance, which means employs a nuclear receptor-cofactor system, exhibits detection high sensitivity, provides a convenient detection process, and realizes efficient establishment of a detection system.

摘要翻译： 提供了一种检测方法，包括暴露于与辅因子结合的表面接触，用作辅因子的对应物的核受体蛋白质和测试样品; 并且基于核受体蛋白质和辅因子之间的结合程度的变化来检测测试样品中包含的并与核受体结合的物质。 检测方法是用于检测活体相关物质的手段，这意味着采用核受体辅因子系统，具有高灵敏度的检测，提供方便的检测过程，并且实现了检测系统的有效建立。

公开(公告)号：US20080213799A1

公开(公告)日：2008-09-04

申请号：US11887477

申请日：2005-12-13

申请人： Haruki Mizukami ,  Akira Okuyama ,  Yasuhiko Hatano

发明人： Haruki Mizukami ,  Akira Okuyama ,  Yasuhiko Hatano

IPC分类号： G01N33/53

CPC分类号： G01N33/743 ,  G01N2333/723 ,  G01N2500/04

摘要： Provided is a detection method including exposing, to contact with a surface to which a cofactor has been bound, a nuclear receptor protein serving as a counterpart of the cofactor and a test sample; and detecting a substance which is contained in the test sample and which binds to the nuclear receptor, on the basis of a change in degree of binding between the nuclear receptor protein and the cofactor. The detection method is means for detecting a living-body-related substance, which means employs a nuclear receptor-cofactor system, exhibits detection high sensitivity, provides a convenient detection process, and realizes efficient establishment of a detection system.

摘要翻译： 提供了一种检测方法，包括暴露于与辅因子结合的表面接触，用作辅因子的对应物的核受体蛋白质和测试样品; 并且基于核受体蛋白质和辅因子之间的结合程度的变化来检测测试样品中包含的并与核受体结合的物质。 检测方法是用于检测活体相关物质的手段，这意味着采用核受体辅因子系统，具有高灵敏度的检测，提供方便的检测过程，并且实现了检测系统的有效建立。

公开(公告)号：US20090269857A1

公开(公告)日：2009-10-29

申请号：US12458235

申请日：2009-07-06

申请人： Haruki Mizukami ,  Kazuto Nishi

发明人： Haruki Mizukami ,  Kazuto Nishi

IPC分类号： G01N33/558 ,  G01N33/00 ,  G01N21/00

CPC分类号： G01N33/5308 ,  G01N33/555 ,  G01N33/558

摘要： To provide the following instruments 1 and 2 as a low-molecular-weight substance detection instrument employing immunochromatography capable of detecting conveniently and sensitively detecting a low-molecular weight substance such as an environmental pollutant (e.g., a dioxin), as a target substance contained in a test sample: 1. an instrument, which comprises 1) a test sample application section with which a test sample is brought into contact; 2) a label product reaction section containing a label product containing, as a portion thereof, an antibody capable of binding to a target substance contained in the test sample, the label product being not bound to the reaction section; 3) an unbound label product capturing section containing an element capable of capturing the label product which is not bound to the target substance, the element being bound to the capturing section; and 4) a detection section containing a detection element which, when coming into contact with the target substance bound to the label product, causes a visually observable change, and 2. an instrument wherein a test sample is reacted with a labeled antibody containing, as a portion thereof, an antibody capable of binding to a target substance contained in the test sample, and the resultant reaction product is employed for detecting the target substance contained in the test sample.

摘要翻译： 为了提供以下仪器1和2作为使用能够方便地检测低分子量物质如环境污染物（例如二恶英）的免疫色谱的低分子量物质检测仪器作为目标物质，其包含 在测试样品中：1.仪器，其包括1）与测试样品接触的测试样品施加部分; 2）一种标签产物反应部分，其含有标签产物，该标签产物作为其一部分含有能够结合试验样品中所含目标物质的抗体，标记产物不与反应部分结合; 3）一种未绑定的标签产品捕获部分，其包含能够捕获不与目标物质结合的标签产品的元件，该元件被绑定到捕获部分; 以及4）检测部，其含有检测元件，所述检测元件在与标记物结合的目标物质接触时引起视觉上可观察的变化，以及2.将检测样品与标记抗体反应的仪器，所述标记抗体含有 其部分，能够结合试验样品中所含的目标物质的抗体，所得反应产物用于检测试验样品中所含的目标物质。

公开(公告)号：US20050148097A1

公开(公告)日：2005-07-07

申请号：US10506807

申请日：2003-03-06

申请人： Haruki Mizukami

发明人： Haruki Mizukami

IPC分类号： G01N33/53 ,  G01N33/555 ,  G01N33/558

CPC分类号： G01N33/5308 ,  G01N33/555 ,  G01N33/558

摘要： To provide the following instruments 1 and 2 as a low-molecular-weight substance detection instrument employing immunochromatography capable of detecting conveniently and sensitively detecting a low-molecular weight substance such as an environmental pollutant (e.g., a dioxin), as a target substance contained in a test sample: 1. an instrument, which comprises 1) a test sample application section with which a test sample is brought into contact; 2) a label product reaction section containing a label product containing, as a portion thereof, an antibody capable of binding to a target substance contained in the test sample, the label product being not bound to the reaction section; 3) an unbound label product capturing section containing an element capable of capturing the label product which is not bound to the target substance, the element being bound to the capturing section; and 4) a detection section containing a detection element which, when coming into contact with the target substance bound to the label product, causes a visually observable change, and 2. an instrument wherein a test sample is reacted with a labeled antibody containing, as a portion thereof, an antibody capable of binding to a target substance contained in the test sample, and the resultant reaction product is employed for detecting the target substance contained in the test sample.

摘要翻译： 为了提供以下仪器1和2作为使用能够方便地检测低分子量物质如环境污染物（例如二恶英）的免疫色谱的低分子量物质检测仪器作为目标物质，其包含 在测试样品中：1.仪器，其包括1）与测试样品接触的测试样品施加部分; 2）一种标签产物反应部分，其含有标签产物，该标签产物作为其一部分含有能够结合试验样品中所含目标物质的抗体，标记产物不与反应部分结合; 3）一种未绑定的标签产品捕获部分，其包含能够捕获不与目标物质结合的标签产品的元件，该元件被绑定到捕获部分; 以及4）检测部，其含有检测元件，所述检测元件在与标记物结合的目标物质接触时引起视觉上可观察的变化，以及2.将检测样品与标记抗体反应的仪器，所述标记抗体含有 其部分，能够结合试验样品中所含的目标物质的抗体，所得反应产物用于检测试验样品中所含的目标物质。