公开(公告)号：US20090107909A1

公开(公告)日：2009-04-30

申请号：US12227400

申请日：2007-05-22

申请人： Hidetoshi Kotera ,  Masao Washizu ,  Hidehiro Oana

发明人： Hidetoshi Kotera ,  Masao Washizu ,  Hidehiro Oana

IPC分类号： B01D17/025

CPC分类号： G01N33/491 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0809 ,  B01L2300/0864 ,  B01L2400/0457 ,  B01L2400/086 ,  Y10T137/2082 ,  Y10T137/212 ,  Y10T137/218

摘要： It is intended to provide a microchannel for separating blood plasma whereby blood plasma can be conveniently separated on a microchip.A microchannel comprising: a substrate body (10a); a separation channel (11) which is formed within the substrate body (10a) and extended in the direction parallel to the longitudinal direction of the substrate body (10a); at least two (first and second) connection ports (13a, 14a) which are formed at one end of the separation channel (11) close to the thickness direction perpendicular to the longitudinal direction of the substrate body (10a); and first and second branch channels (13, 14) which are formed within the substrate body (10a) and connected respectively to the first and second connection ports (13a, 14a). In the state where the substrate body (10a) is extended perpendicular to the direction of gravitational force, a blood sample containing blood cells and plasma is flown from one end (11a) side of the separation channel (11). Before the blood sample attains the other end (11b) of the separation channel (11), the blood cells in the blood sample settle out. The blood cells having settled out are then introduced into the branch channel (14) that is located lower in the direction of gravitational force between the first and second branch channels. On the other hand, the major portion of the blood plasma is introduced into the other branch channel (13) located upper in the direction of gravitational force.

摘要翻译： 旨在提供用于分离血浆的微通道，从而可以在微芯片上方便地分离血浆。 一种微通道，包括：衬底主体（10a）; 分离通道（11），其形成在所述基板主体（10a）内并沿与所述基板主体（10a）的纵向方向平行的方向延伸; 至少两个（第一和第二）连接端口（13a，14a），其形成在与所述基板主体（10a）的纵向方向垂直的厚度方向上的分离通道（11）的一端处; 以及第一和第二分支通道（13,14），其形成在所述基板主体（10a）内并分别连接到所述第一和第二连接端口（13a，14a）。 在基板主体（10a）垂直于重力方向延伸的状态下，含有血细胞和血浆的血液样本从分离通道（11）的一端（11a）侧流出。 在血液样本达到分离通道（11）的另一端（11b）之前，血液样品中的血细胞沉降。 然后将沉淀出的血细胞引入位于第一和第二分支通道之间的重力方向下方较低的分支通道（14）中。 另一方面，等离子体的主要部分被引入位于重力方向上方的另一个分支通道（13）中。

公开(公告)号：US08003062B2

公开(公告)日：2011-08-23

申请号：US12227400

申请日：2007-05-22

申请人： Hidetoshi Kotera ,  Masao Washizu ,  Hidehiro Oana

发明人： Hidetoshi Kotera ,  Masao Washizu ,  Hidehiro Oana

IPC分类号： B01D17/025

CPC分类号： G01N33/491 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0809 ,  B01L2300/0864 ,  B01L2400/0457 ,  B01L2400/086 ,  Y10T137/2082 ,  Y10T137/212 ,  Y10T137/218

摘要： A microchannel has a substrate body; a separation channel which is formed within the substrate; at least two (first and second) connection ports which are formed at one end of the separation channel close to the thickness direction perpendicular to the longitudinal direction of the substrate body; and first and second branch channels connected respectively to the first and second connection ports. When the substrate body is perpendicular to gravity, a blood sample containing blood cells and plasma, flows from one end of the separation channel. Before the blood sample gets to the other end of the separation channel, the blood cells in the blood sample settle out. The blood cells having settled out are then separated into the branch channel.

摘要翻译： 微通道具有基体; 在衬底内形成的分离通道; 至少两个（第一和第二）连接端口，其形成在分离通道的一端，靠近垂直于基板主体的纵向方向的厚度方向; 以及分别连接到第一和第二连接端口的第一和第二分支通道。 当衬底主体垂直于重力时，含有血细胞和血浆的血液样本从分离通道的一端流出。 在血液样本进入分离通道的另一端之前，血液样本中的血细胞沉降。 然后将沉淀的血细胞分离成分支通道。