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    MICROFLUIDIC DEVICE FOR MANIPULATING A DISCRETE ELEMENT
    4.
    发明公开
    MICROFLUIDIC DEVICE FOR MANIPULATING A DISCRETE ELEMENT 审中-公开

    公开(公告)号：US20240316559A1

    公开(公告)日：2024-09-26

    申请号：US18574175

    申请日：2021-07-15

    申请人： UNIVERSITÉ DE LIÈGE

    发明人： Stéphanie VAN LOO ,  Tristan GILET

    IPC分类号： B01L3/00

    CPC分类号： B01L3/502792 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0864 ,  B01L2400/086

    摘要： A microfluidic device for use in field of droplet microfluidics is disclosed. The microfluidic device can manipulate a discrete element, for example a droplet. The discrete element may include a medium and a component. The microfluidic device may include a main microfluidic channel, some stopping elements and an attractive mechanism that may retain, physically and in a releasable way, the component at a given location in the main microfluidic channel. The discrete element may be split into a first and second parts in such a way that the component ends in the second parts. The microfluidic device. may be used especially for a single-cell analysis.

    BIOLOGICAL SUBSTANCE DETECTION METHOD USING WELL ARRAY AND PARTICLES, WELL ARRAY, AND DETECTION DEVICE
    5.
    发明公开
    BIOLOGICAL SUBSTANCE DETECTION METHOD USING WELL ARRAY AND PARTICLES, WELL ARRAY, AND DETECTION DEVICE 审中-公开

    公开(公告)号：US20240302371A1

    公开(公告)日：2024-09-12

    申请号：US18552371

    申请日：2022-03-04

    申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

    发明人： Makoto Fujimaki ,  Hiroki Ashiba

    IPC分类号： G01N33/569 ,  B01L3/00 ,  G01N33/543

    CPC分类号： G01N33/56983 ,  B01L3/5085 ,  G01N33/54386 ,  B01L2200/0652 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2300/0829 ,  G01N2469/10

    摘要： To detect a biological substance at high speed and high sensitivity by using a well array and particles. The biological substance detection method of the present invention includes a step of preparing a test liquid containing particles at a concentration of at least 5×107 particles/mL, a step of allowing the particles to trap the biological substance to form a trapped body, a step of sending the test liquid onto the well array to store a plurality of the particles including the trapped body in the wells while storing the number of the particles to be stored in one well to a minimum average storage number Nmin represented by the following formulas (1) and (2), and a step of detecting the color development of the well with an image pickup element.







    [

    Mathematical
    ⁢

    Formula
    ⁢

    1

    ]










    N
    min

    =

    C
    ×

    V
    L

    ×


    P
    image


    P
    total







    (
    1
    )













    N
    min

    ≧
    8




    (
    2
    )







    In the formula (1), C represents a concentration of the particles in the test liquid; VL represents an amount of the test liquid and is at least 5 μL; Ptotal represents the number of pixels of the image pickup element and is at least 300,000 pixels; and Pimage represents the number of pixels of a pixel group used for imaging one of the wells in the image pickup element and is at least 9 pixels.

    LABELING BIOLOGICAL PARTICLES USING ACOUSTOPHORESIS
    7.
    发明公开
    LABELING BIOLOGICAL PARTICLES USING ACOUSTOPHORESIS 审中-公开

    公开(公告)号：US20240288424A1

    公开(公告)日：2024-08-29

    申请号：US18290442

    申请日：2022-05-11

    申请人： THE UNITED STATES OF AMERICA, as Represented by the Secretary, Department of Health and Humag

    发明人： Stephen S. Adler ,  Noriko Sato ,  Peter Choyke

    IPC分类号： G01N33/569 ,  B01L3/00

    CPC分类号： G01N33/56972 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0663 ,  B01L2300/0864 ,  B01L2400/0436

    摘要： Methods and apparatus are described for labeling biological particles using acoustophoresis. In a washing stage, two adjacent substreams are passed through a region having a gradient in acoustic energy density, leading to particles being driven from one substream to the other. Washed bioparticles flowing in a medium are accumulated at a trapping site by an acoustic energy barrier while the medium passes by. Accumulated particles are discharged in batches and mixed with labeling particles. The mixture is incubated in a chamber, producing labeled biological particles, which are washed in another washing stage. Processing operations are automated e.g. on a microfluidics platform. Diagnostic and therapeutic applications are disclosed, covering a range of particle types and a range of labeling types. Exemplary biological particles include cells, antibodies, or fragments thereof. Exemplary labeling particles have detection functions (radioligand, magnetic particles, fluorophores), therapeutic functions (drugs), or binding functions (adhesion molecules).