公开(公告)号：US20130280807A1

公开(公告)日：2013-10-24

申请号：US13884651

申请日：2011-11-10

申请人： Toshiaki Takezawa ,  Hiroyuki Yamaguchi ,  Hiroyuki Kuroyama ,  Tomoya Sawaguchi

发明人： Toshiaki Takezawa ,  Hiroyuki Yamaguchi ,  Hiroyuki Kuroyama ,  Tomoya Sawaguchi

IPC分类号： G01N33/50

CPC分类号： G01N33/5008 ,  C12M21/08 ,  C12M23/10 ,  C12M25/04 ,  C12M25/14

摘要： A single cell-culture chamber is provided that includes a dried vitrigel membrane covering and secured to one open end surface of a tubular frame. Also provided is a double cell-culture chamber that includes two tubular frames of substantially the same planar cross-sectional shape adhesively secured to each other with a dried vitrigel membrane interposed between the opposing open end surfaces of the tubular frames so as to form a first chamber and a second chamber via the dried vitrigel membrane.

摘要翻译： 提供单个细胞培养室，其包括覆盖并固定到管状框架的一个开放端表面的干燥的玻璃质凝胶膜。 还提供了一种双细胞培养室，其包括两个基本上相同的平面截面形状的管状框架，其通过插入在管状框架的相对开口端表面之间的干燥玻璃凝胶膜彼此粘合地固定，以形成第一 室和通过干燥的玻璃体膜的第二室。

公开(公告)号：US20130217126A1

公开(公告)日：2013-08-22

申请号：US13818759

申请日：2011-08-25

申请人： Toshiaki Takezawa ,  Ayumi Oshikata ,  Hiroyuki Kuroyama ,  Tomoya Sawaguchi ,  Hiroyuki Yamaguchi

发明人： Toshiaki Takezawa ,  Ayumi Oshikata ,  Hiroyuki Kuroyama ,  Tomoya Sawaguchi ,  Hiroyuki Yamaguchi

IPC分类号： C12N5/00 ,  B29D7/01

CPC分类号： B29D7/01 ,  B29C39/02 ,  B29C41/02 ,  B29K2089/00 ,  C12M25/14 ,  C12N5/0062

摘要： A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold; (2) a step of removing the wall surface mold from the top of the substrate; (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel; (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.

摘要翻译： 干燥玻璃凝胶膜是通过以下步骤制造的：（1）将水凝胶保持在壁表面模具内部的形状与设置在基板上的所需形状相同的形状的步骤， 水凝胶内的自由水从基材和壁表面模具之间的间隙中释放出来; （2）从基板的顶部除去壁面模具的工序; （3）干燥水凝胶以去除残留游离水的步骤，由此制造玻璃化干燥水凝胶; （4）将干燥的水凝胶再水化以制造玻璃el膜的步骤; 和（5）重新搅拌玻璃浆膜以除去游离水的步骤，从而制造玻璃化的干燥玻璃凝胶膜。