公开(公告)号：US20140237688A1

公开(公告)日：2014-08-21

申请号：US14234007

申请日：2012-07-20

Applicant: Yin-Fu Chang ,  Aparna Sri Vanguri ,  Leslie Grist ,  Holly Tuttle ,  Hai Ping Hong ,  Paula Olhoft

Inventor： Yin-Fu Chang ,  Aparna Sri Vanguri ,  Leslie Grist ,  Holly Tuttle ,  Hai Ping Hong ,  Paula Olhoft

IPC: C12N15/82

Abstract: The present invention relates to an improved method of producing a transgenic plant. Said method comprises, inter alia, the steps of a) providing a wounded transformable explant comprising a hypocotyl or a portion thereof, at least one cotyledon and wounded tissue, b) transforming cells comprised by said explant, and c) transferring said explant to a growing medium, comprising at least one selection compound for a selectable marker, by inserting the hypocotyl of said explant into said growing medium. Moreover, the present invention relates to a plant obtainable by the method according to the present invention.

Abstract translation: 本发明涉及生产转基因植物的改进方法。 所述方法尤其包括以下步骤：a）提供包含下胚轴或其部分，至少一个子叶和受伤组织的受伤可转化外植体，b）转化由所述外植体组成的细胞，和c）将所述外植体转移至 通过将所述外植体的下胚轴插入所述生长培养基中，包含用于选择性标记的至少一种选择化合物。 此外，本发明涉及通过本发明的方法获得的植物。

公开(公告)号：US10557141B2

公开(公告)日：2020-02-11

申请号：US14234007

申请日：2012-07-20

Applicant: Yin-Fu Chang ,  Aparna Sri Vanguri ,  Leslie Grist ,  Holly Tuttle ,  Hai Ping Hong ,  Paula Olhoft

Inventor： Yin-Fu Chang ,  Aparna Sri Vanguri ,  Leslie Grist ,  Holly Tuttle ,  Hai Ping Hong ,  Paula Olhoft

IPC: C12N15/82

Abstract: The present invention relates to an improved method of producing a transgenic plant. Said method comprises, inter alia, the steps of a) providing a wounded transformable explant comprising a hypocotyl or a portion thereof, at least one cotyledon and wounded tissue, b) transforming cells comprised by said explant, and c) transferring said explant to a growing medium, comprising at least one selection compound for a selectable marker, by inserting the hypocotyl of said explant into said growing medium. Moreover, the present invention relates to a plant obtainable by the method according to the present invention.