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    Plant Transformation Method
    1.
    发明申请
    Plant Transformation Method 审中-公开
    植物转化方法

    公开(公告)号：US20140237688A1

    公开(公告)日：2014-08-21

    申请号：US14234007

    申请日：2012-07-20

    申请人： Yin-Fu Chang ,  Aparna Sri Vanguri ,  Leslie Grist ,  Holly Tuttle ,  Hai Ping Hong ,  Paula Olhoft

    发明人： Yin-Fu Chang ,  Aparna Sri Vanguri ,  Leslie Grist ,  Holly Tuttle ,  Hai Ping Hong ,  Paula Olhoft

    IPC分类号： C12N15/82

    摘要： The present invention relates to an improved method of producing a transgenic plant. Said method comprises, inter alia, the steps of a) providing a wounded transformable explant comprising a hypocotyl or a portion thereof, at least one cotyledon and wounded tissue, b) transforming cells comprised by said explant, and c) transferring said explant to a growing medium, comprising at least one selection compound for a selectable marker, by inserting the hypocotyl of said explant into said growing medium. Moreover, the present invention relates to a plant obtainable by the method according to the present invention.

    摘要翻译： 本发明涉及生产转基因植物的改进方法。 所述方法尤其包括以下步骤：a）提供包含下胚轴或其部分，至少一个子叶和受伤组织的受伤可转化外植体，b）转化由所述外植体组成的细胞，和c）将所述外植体转移至 通过将所述外植体的下胚轴插入所述生长培养基中，包含用于选择性标记的至少一种选择化合物。 此外，本发明涉及通过本发明的方法获得的植物。