公开(公告)号：US08581584B2

公开(公告)日：2013-11-12

申请号：US13067351

申请日：2011-05-26

申请人： Mukesh Sharma ,  Myunggi Yi ,  Hao Dong ,  Huajun Qin ,  David D. Busath ,  Huan-Xiang Zhou ,  Timothy A. Cross

发明人： Mukesh Sharma ,  Myunggi Yi ,  Hao Dong ,  Huajun Qin ,  David D. Busath ,  Huan-Xiang Zhou ,  Timothy A. Cross

IPC分类号： G01V3/00

CPC分类号： G01N24/088 ,  G01R33/4641

摘要： The invention relates to the atomistic functional understanding of the M2 protein from the influenza A virus. This acid-activated selective proton channel has been the subject of numerous conductance, structural, and computational studies. Previously, little was known at the atomic level about the heart of the functional mechanism of this tetrameric protein, a tetrad of HxxxW residues. The structure of the M2 conductance domain in a lipid bilayer is disclosed and displays the defining features of the native protein that have not been attainable from structures solubilized by detergents. A detailed mechanism for acid activation and proton conductance, involving a strong hydrogen bond between two adjacent histidines and specific interactions with the tryptophan gate, is provided and elucidates many observations on the M2 proton conductance.

摘要翻译： 本发明涉及来自甲型流感病毒的M2蛋白的原子功能理解。 这种酸激活的选择性质子通道已经成为许多电导，结构和计算研究的主题。 以前，在原子水平上很少知道这种四聚体蛋白质的四分之一的HxxxW残基的功能机制的心脏。 公开了脂质双层中的M2电导结构域的结构，并显示了通过洗涤剂溶解的结构不能获得的天然蛋白质的定义特征。 提供了一个详细的酸活化和质子传导机制，涉及两个相邻组氨酸之间的强氢键和与色氨酸门的特异性相互作用，并阐明了许多对M2质子传导的观察。

公开(公告)号：US20110291652A1

公开(公告)日：2011-12-01

申请号：US13067351

申请日：2011-05-26

申请人： Mukesh Sharma ,  Myunggi Yi ,  Hao Dong ,  Huajun Qin ,  David D. Busath ,  Huan-Xiang Zhou ,  Timothy A. Cross

发明人： Mukesh Sharma ,  Myunggi Yi ,  Hao Dong ,  Huajun Qin ,  David D. Busath ,  Huan-Xiang Zhou ,  Timothy A. Cross

IPC分类号： G01R33/44

CPC分类号： G01N24/088 ,  G01R33/4641

摘要： The invention relates to the atomistic functional understanding of the M2 protein from the influenza A virus. This acid-activated selective proton channel has been the subject of numerous conductance, structural, and computational studies. Previously, little was known at the atomic level about the heart of the functional mechanism of this tetrameric protein, a tetrad of HxxxW residues. The structure of the M2 conductance domain in a lipid bilayer is disclosed and displays the defining features of the native protein that have not been attainable from structures solubilized by detergents. A detailed mechanism for acid activation and proton conductance, involving a strong hydrogen bond between two adjacent histidines and specific interactions with the tryptophan gate, is provided and elucidates many observations on the M2 proton conductance.

摘要翻译： 本发明涉及来自甲型流感病毒的M2蛋白的原子功能理解。 这种酸激活的选择性质子通道已经成为许多电导，结构和计算研究的主题。 以前，在原子水平上很少知道这种四聚体蛋白质的四分之一的HxxxW残基的功能机制的心脏。 公开了脂质双层中的M2电导结构域的结构，并显示了通过洗涤剂溶解的结构不能获得的天然蛋白质的定义特征。 提供了一个详细的酸活化和质子传导机制，涉及两个相邻组氨酸之间的强氢键和与色氨酸门的特异性相互作用，并阐明了许多对M2质子传导的观察。