公开(公告)号：US20110165652A1

公开(公告)日：2011-07-07

申请号：US12812952

申请日：2009-01-14

申请人： Susan H. Hardin ,  Tommie Lloyd Lincecum, JR. ,  Norha Deluge ,  Hongyi Wang ,  Yuri Belosludtsev ,  Kristi Kincaid ,  Anelia Kraltcheva ,  Benjamin Stevens ,  Ming Fa ,  Amy Bryant ,  Amy Castillo ,  Hye Eun Kim ,  Uma Nagaswamy ,  Mitsu Sreedhar Reddy ,  Alok N. Bandekar ,  Ivan Pan ,  Andrei Volkov

发明人： Susan H. Hardin ,  Tommie Lloyd Lincecum, JR. ,  Norha Deluge ,  Hongyi Wang ,  Yuri Belosludtsev ,  Kristi Kincaid ,  Anelia Kraltcheva ,  Benjamin Stevens ,  Ming Fa ,  Amy Bryant ,  Amy Castillo ,  Hye Eun Kim ,  Uma Nagaswamy ,  Mitsu Sreedhar Reddy ,  Alok N. Bandekar ,  Ivan Pan ,  Andrei Volkov

IPC分类号： C12N9/12

CPC分类号： C07H19/207 ,  C07H19/10 ,  C12N9/1252 ,  C12Q1/6869

摘要： Compositions, systems and methods of sequencing are disclosed, where the compositions and systems include polymerase enzymes that have been genetically modified to more efficiently incorporate nucleotides including labels having a detectable properties that are released during incorporation, to augment a rate of labeled nucleotide incorporation, to augment a rate of pyrophosphate release, or to augment two or more of these properties and rates. Also disclosed are terminally labeled and dual labeled nucleotides, and click-chemistry based methods of synthesizing the same.

摘要翻译： 公开了组合物，系统和测序方法，其中组合物和系统包括经遗传修饰以更有效地掺入核苷酸的聚合酶，包括在掺入期间释放的具有可检测性质的标记，以增加标记的核苷酸掺入率， 增加焦磷酸盐释放的速率，或增加这些性质和速率中的两种或更多种。 还公开了末端标记和双重标记的核苷酸，以及基于点击化学的合成方法。