公开(公告)号：US20110165652A1

公开(公告)日：2011-07-07

申请号：US12812952

申请日：2009-01-14

申请人： Susan H. Hardin ,  Tommie Lloyd Lincecum, JR. ,  Norha Deluge ,  Hongyi Wang ,  Yuri Belosludtsev ,  Kristi Kincaid ,  Anelia Kraltcheva ,  Benjamin Stevens ,  Ming Fa ,  Amy Bryant ,  Amy Castillo ,  Hye Eun Kim ,  Uma Nagaswamy ,  Mitsu Sreedhar Reddy ,  Alok N. Bandekar ,  Ivan Pan ,  Andrei Volkov

发明人： Susan H. Hardin ,  Tommie Lloyd Lincecum, JR. ,  Norha Deluge ,  Hongyi Wang ,  Yuri Belosludtsev ,  Kristi Kincaid ,  Anelia Kraltcheva ,  Benjamin Stevens ,  Ming Fa ,  Amy Bryant ,  Amy Castillo ,  Hye Eun Kim ,  Uma Nagaswamy ,  Mitsu Sreedhar Reddy ,  Alok N. Bandekar ,  Ivan Pan ,  Andrei Volkov

IPC分类号： C12N9/12

CPC分类号： C07H19/207 ,  C07H19/10 ,  C12N9/1252 ,  C12Q1/6869

摘要： Compositions, systems and methods of sequencing are disclosed, where the compositions and systems include polymerase enzymes that have been genetically modified to more efficiently incorporate nucleotides including labels having a detectable properties that are released during incorporation, to augment a rate of labeled nucleotide incorporation, to augment a rate of pyrophosphate release, or to augment two or more of these properties and rates. Also disclosed are terminally labeled and dual labeled nucleotides, and click-chemistry based methods of synthesizing the same.

摘要翻译： 公开了组合物，系统和测序方法，其中组合物和系统包括经遗传修饰以更有效地掺入核苷酸的聚合酶，包括在掺入期间释放的具有可检测性质的标记，以增加标记的核苷酸掺入率， 增加焦磷酸盐释放的速率，或增加这些性质和速率中的两种或更多种。 还公开了末端标记和双重标记的核苷酸，以及基于点击化学的合成方法。

公开(公告)号：US20090186343A1

公开(公告)日：2009-07-23

申请号：US12035352

申请日：2008-02-21

申请人： Hongyi Wang ,  Norha Deluge ,  Kristi Kincaid ,  Yuri Belosludtsev ,  Tommie Lincecum, JR. ,  Amy Williams ,  Amy Bryant ,  Ming Fa ,  Benjamin Stevens ,  Susan H. Hardin

发明人： Hongyi Wang ,  Norha Deluge ,  Kristi Kincaid ,  Yuri Belosludtsev ,  Tommie Lincecum, JR. ,  Amy Williams ,  Amy Bryant ,  Ming Fa ,  Benjamin Stevens ,  Susan H. Hardin

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C07H1/00 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/6869 ,  C12Q2565/101 ,  C12Q2525/117 ,  C12Q2521/101

摘要： A novel and efficient single pot synthetic schemes are disclosed for preparing modified nucleotides, nucleotide analogs, nucleotide polyphosphates, and nucleotide polyphosphate analogs. The novel method is used to prepare nucleotides, nucleotide analogs, nucleotide polyphosphates, and nucleotide polyphosphate analogs having non-persistent or persistent and non-persistent modifications. Novel biomolecular reactions are also disclosed using the novel modified biomolecules disclosed herein.

摘要翻译： 公开了一种用于制备修饰的核苷酸，核苷酸类似物，核苷酸多磷酸酯和核苷酸多磷酸酯类似物的新型和有效的单锅合成方案。 该新方法用于制备具有非持久性或持久性和非持久性修饰的核苷酸，核苷酸类似物，核苷酸多磷酸酯和核苷酸多磷酸酯类似物。 使用本文公开的新的改性生物分子也公开了新的生物分子反应。

公开(公告)号：US20100323110A1

公开(公告)日：2010-12-23

申请号：US12852356

申请日：2010-08-06

申请人： Yuri BELOSLUDTSEV ,  Nasanshargal BATTULGA ,  Mitsu REDDY ,  Anelia KRALTCHEVA ,  Susan H. HARDIN ,  Tommie L. LINCECUM, JR. ,  Hongyi WANG ,  Norha DELUGE ,  Uma NAGASWAMY ,  Benjamin C. STEVENS ,  Kristi K. KINCAID

发明人： Yuri BELOSLUDTSEV ,  Nasanshargal BATTULGA ,  Mitsu REDDY ,  Anelia KRALTCHEVA ,  Susan H. HARDIN ,  Tommie L. LINCECUM, JR. ,  Hongyi WANG ,  Norha DELUGE ,  Uma NAGASWAMY ,  Benjamin C. STEVENS ,  Kristi K. KINCAID

IPC分类号： B05D3/10

CPC分类号： G01N21/6428

摘要： Support surfaces are disclosed that are designed to support molecules or molecular assemblies immobilized thereon so that the molecules or molecular assemblies can be observed in single molecule detections systems, where the support surfaces have reduced background and the fluorescent labels associated with the immobilized molecules or molecular assemblies have longer active lifetimes prior to permanent photo-bleaching or deactivation and have improve fluorescence properties and where the surfaces have more uniform fluorescent properties.

摘要翻译： 公开了支撑表面，其被设计成支持固定在其上的分子或分子组件，使得可以在单分子检测系统中观察分子或分子组件，其中支持表面具有降低的背景，并且与固定化分子或分子组装相关联的荧光标记 在永久性光漂白或去活化之前具有更长的活性寿命，并且具有改善的荧光性能以及其表面具有更均匀的荧光性质。

公开(公告)号：US20080091005A1

公开(公告)日：2008-04-17

申请号：US11781160

申请日：2007-07-20

申请人： Hongyi Wang ,  Xiaolian Gao ,  Peilin Yu ,  Mitsu Reddy ,  Susan Hardin ,  Tommie Lincecum ,  Amy Williams ,  Norha Deluge ,  Yuri Belosludtsev ,  Steven Menchen ,  Joe Lam ,  Jer-Kang Chen

发明人： Hongyi Wang ,  Xiaolian Gao ,  Peilin Yu ,  Mitsu Reddy ,  Susan Hardin ,  Tommie Lincecum ,  Amy Williams ,  Norha Deluge ,  Yuri Belosludtsev ,  Steven Menchen ,  Joe Lam ,  Jer-Kang Chen

IPC分类号： C07H21/04

CPC分类号： C07H21/04

摘要： Modified nucleotides are disclosed for use in single molecule sequencing, methods for making the modified nucleotides and method for using the modified nucleotides. Linkers for making the modified nucleotide are also disclosed.

摘要翻译： 公开了用于单分子测序的修饰的核苷酸，用于制备修饰的核苷酸的方法和使用修饰的核苷酸的方法。 还公开了用于制备修饰的核苷酸的接头。

公开(公告)号：US20080076189A1

公开(公告)日：2008-03-27

申请号：US11694605

申请日：2007-03-30

申请人： Yuri Belosludtsev ,  Nasanshargal Battulga ,  Mistu Reddy ,  Anelia Kraltcheva ,  Susan Hardin ,  Tommie Lincecum ,  Hongyi Wang ,  Norha Deluge ,  Uma Nagaswamy ,  Benjamin Stevens ,  Kristi Kincaid

发明人： Yuri Belosludtsev ,  Nasanshargal Battulga ,  Mistu Reddy ,  Anelia Kraltcheva ,  Susan Hardin ,  Tommie Lincecum ,  Hongyi Wang ,  Norha Deluge ,  Uma Nagaswamy ,  Benjamin Stevens ,  Kristi Kincaid

IPC分类号： G01N21/64

CPC分类号： G01N21/6428

摘要： Support surfaces are disclosed that are designed to support molecules or molecular assemblies immobilized thereon so that the molecules or molecular assemblies can be observed in single molecule detections systems, where the support surfaces have reduced background and the fluorescent labels associated with the immobilized molecules or molecular assemblies have longer active lifetimes prior to permanent photo-bleaching or deactivation and have improve fluorescence properties and where the surfaces have more uniform fluorescent properties.

摘要翻译： 公开了支撑表面，其被设计成支持固定在其上的分子或分子组件，使得可以在单分子检测系统中观察分子或分子组件，其中支持表面具有降低的背景，并且与固定化分子或分子组装相关联的荧光标记 在永久性光漂白或去活化之前具有更长的活性寿命，并且具有改善的荧光性能以及其表面具有更均匀的荧光性质。