公开(公告)号：US20190127730A1

公开(公告)日：2019-05-02

申请号：US16096267

申请日：2017-04-25

申请人： Idea Orchard, LLC

发明人： Rajika Perera ,  Michael Longo

IPC分类号： C12N15/10

摘要： A binding agent to a target molecule, or method or kit where the binding agent is selected from a library where each variant has a circular permutation of the FHA domain where the rearrange does not substantially disrupt the FHA domain's beta-sheet scaffold or increase the stability of the beta-sheet scaffold. The randomized regions of the FHA domain include the endogenous binding interface the FHA domain, the region opposite of the endogenous binding interface, and the circular permutation region.

公开(公告)号：US20190316275A1

公开(公告)日：2019-10-17

申请号：US16445121

申请日：2019-06-18

申请人： IDEA ORCHARD, LLC

发明人： Michael Longo ,  Rajika Perera

IPC分类号： C40B40/10 ,  C12N15/10 ,  C40B10/00 ,  C40B40/08

摘要： A general method and recombinant nucleic acid sequences, by means which the method selects a recombinant protein containing an FHA domain for binding a target molecule from a library proteins with a high-throughput method of creating protein variations within the FHA domain in non-conserved or non-structural sequences of the FHA scaffold, and the library may also be in the form of a phagemid or phage library wherein the ALP nucleic acid sequence is inserted into a vector capable of allowing the vector and expressed ALP protein from being virally packaged, and the recombinant nucleic acid sequences which are randomly mutated at varying non-conserved or non-structural FHA domain sequences.

公开(公告)号：US11214791B2

公开(公告)日：2022-01-04

申请号：US16096267

申请日：2017-04-25

申请人： Idea Orchard, LLC

发明人： Rajika Perera ,  Michael Longo

IPC分类号： C40B40/10 ,  C12N15/10 ,  C07K14/47

摘要： A binding agent to a target molecule, or method or kit where the binding agent is selected from a library where each variant has a circular permutation of the FHA domain where the rearrange does not substantially disrupt the FHA domain's beta-sheet scaffold or increase the stability of the beta-sheet scaffold. The randomized regions of the FHA domain include the endogenous binding interface the FHA domain, the region opposite of the endogenous binding interface, and the circular permutation region.