公开(公告)号：US06790951B1

公开(公告)日：2004-09-14

申请号：US09068507

申请日：1998-07-15

申请人： Vincent G. H. Eijsink ,  Ingolf F. Nes ,  May B. Brurberg

发明人： Vincent G. H. Eijsink ,  Ingolf F. Nes ,  May B. Brurberg

IPC分类号： C07H2104

CPC分类号： C12N15/746 ,  C07K14/335

摘要： The present invention concerns the discovery of a new regulatory mechanism for gene expression in lactic acid bacteria (LAB), especially Lactobacillus sake LTH673 or Lactobacillus plantarium C11, that includes previously unrecognized strongly regulable promoter elements. The essential finding is that the expression of genes under the control of a promoter element dependent on the expression of the IF-K-R gene cluster. The expression of the IF-K-R gene cluster is autoinduced by the secreted peptide encoded by IF, thus providing a regulable expression system for a desired protein. The invention further comprises the purified IF protein.

摘要翻译： 本发明涉及发现乳酸菌（LAB）中的基因表达的新调控机制，特别是莱茵酸乳杆菌LTH673或植物乳杆菌C11，其包括先前未被认可的强调节的启动子元件。 基本发现是依赖于IF-K-R基因簇的表达的启动子元件控制下的基因的表达。 IF-K-R基因簇的表达由IF编码的分泌肽自动诱导，从而为所需蛋白提供可调节的表达系统。 本发明还包括纯化的IF蛋白。