摘要:
The present invention provides an aptamer capable of specifically adsorbing to bisphenol A suspected to be an endocrine disrupter as a target molecule, a method for obtaining an aptamer capable of specifically adsorbing to bisphenol A by an in vitro selection method utilizing affinity chromatography using a carrier immobilizing bisphenol A, particularly, a method including use of an antagonistic elution buffer containing an amphiprotic organic solvent for elution by the affinity chromatography, and a single-strand nucleic acid molecule which is an aptamer obtained thereby.
摘要:
The present invention provides a method for obtaining an aptamer capable of specifically adsorbing to Verotoxin-1 produced by Enterohemorrhagic Escherichia coli by an in vitro selection method utilizing affinity chromatography, particularly, a method including use of an affinity column immobilizing thereon a peptide having an amino acid sequence of Verotoxin-1, for the affinity chromatography, and a single strand nucleic acid molecule, which is an aptamer capable of specifically adsorbing to Verotoxin-1.