Electro-blotting devices, systems, and kits, and methods for their use

    公开(公告)号:US08268149B2

    公开(公告)日:2012-09-18

    申请号:US11465850

    申请日:2006-08-21

    IPC分类号: G01N27/447

    CPC分类号: G01N27/44739

    摘要: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.

    ELECTRO-BLOTTING DEVICES, SYSTEMS, AND KITS AND METHODS FOR THEIR USE
    2.
    发明申请
    ELECTRO-BLOTTING DEVICES, SYSTEMS, AND KITS AND METHODS FOR THEIR USE 审中-公开
    电动灭火装置,系统,工具及其使用方法

    公开(公告)号:US20090026079A1

    公开(公告)日:2009-01-29

    申请号:US12171880

    申请日:2008-07-11

    IPC分类号: B01D61/42

    CPC分类号: G01N27/44739

    摘要: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.

    摘要翻译: 本发明提供了用于干印迹凝胶的干燥电印迹系统,其中该系统包括电印迹转移堆叠,其包含分析凝胶和印迹膜,阳极,与阳极和转移体之间的阳极并置的阳极凝胶基体的主体 叠层,阴极和阴极凝胶基体与阴极和转移叠层之间的阴极并置,其中阳极凝胶基质和阴极凝胶基质各自包含用于电泳转移的离子源。 干电印系统不使用在电印迹之前加入到系统中的任何液体缓冲液(例如当组装传输堆叠时)。 阳极,阴极或两者可以与电源分离,并且作为一次性电极组件的一部分提供,其还包括包括用于电泳转移的离子的凝胶基质体。

    ELECTRO-BLOTTING DEVICES, SYSTEMS, AND KITS AND METHODS FOR THEIR USE
    3.
    发明申请
    ELECTRO-BLOTTING DEVICES, SYSTEMS, AND KITS AND METHODS FOR THEIR USE 有权
    电动灭火装置,系统,工具及其使用方法

    公开(公告)号:US20120211364A1

    公开(公告)日:2012-08-23

    申请号:US13397515

    申请日:2012-02-15

    IPC分类号: G01N33/559 G01N27/447

    CPC分类号: G01N27/44739

    摘要: The invention provides an electroblotting system for blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, an ion source juxtaposed with the anode between the anode and the transfer stack, a cathode, and another ion source juxtaposed with the cathode between the cathode and the transfer stack, in which the each ion source is sufficient for electrophoretic transfer. The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.

    摘要翻译: 本发明提供了一种用于印迹凝胶的电印迹系统,其中该系统包括电印迹转移堆叠,其包含分析凝胶和印迹膜,阳极,与阳极和转印叠层之间的阳极并置的离子源,阴极, 并且另一个离子源与阴极并排在阴极和转移叠层之间,其中每个离子源足以用于电泳转移。 阳极,阴极或两者可以与电源分离,并且作为一次性电极组件的一部分提供,其还包括包括用于电泳转移的离子的凝胶基质体。

    Electro-Blotting Devices, Systems, and Kits, And Methods For Their Use
    4.
    发明申请
    Electro-Blotting Devices, Systems, and Kits, And Methods For Their Use 有权
    电印设备,系统和套件及其使用方法

    公开(公告)号:US20060278531A1

    公开(公告)日:2006-12-14

    申请号:US11465850

    申请日:2006-08-21

    IPC分类号: B01D61/42 G01N27/00

    CPC分类号: G01N27/44739

    摘要: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.

    摘要翻译: 本发明提供了用于干印迹凝胶的干燥电印迹系统,其中该系统包括电印迹转移堆叠,其包含分析凝胶和印迹膜,阳极,与阳极和转移体之间的阳极并置的阳极凝胶基体的主体 叠层,阴极和阴极凝胶基体与阴极和转移叠层之间的阴极并置,其中阳极凝胶基质和阴极凝胶基质各自包含用于电泳转移的离子源。 干电印系统不使用在电印迹之前加入到系统中的任何液体缓冲液(例如当组装传输堆叠时)。 阳极,阴极或两者可以与电源分离,并且作为一次性电极组件的一部分提供,其还包括包括用于电泳转移的离子的凝胶基质体。

    Electro-blotting devices, systems, and kits and methods for their use
    6.
    发明授权
    Electro-blotting devices, systems, and kits and methods for their use 有权
    电印设备,系统,套件及其使用方法

    公开(公告)号:US08608930B2

    公开(公告)日:2013-12-17

    申请号:US13397515

    申请日:2012-02-15

    IPC分类号: G01N27/447

    CPC分类号: G01N27/44739

    摘要: The invention provides an electroblotting system for blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, an ion source juxtaposed with the anode between the anode and the transfer stack, a cathode, and another ion source juxtaposed with the cathode between the cathode and the transfer stack, in which the each ion source is sufficient for electrophoretic transfer. The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.

    摘要翻译: 本发明提供了一种用于印迹凝胶的电印迹系统,其中该系统包括电印迹转移堆叠,其包含分析凝胶和印迹膜,阳极,与阳极和转印叠层之间的阳极并置的离子源,阴极, 并且另一个离子源与阴极并排在阴极和转移叠层之间,其中每个离子源足以用于电泳转移。 阳极,阴极或两者可以与电源分离,并且作为一次性电极组件的一部分提供,其还包括包括用于电泳转移的离子的凝胶基质体。

    Electro-blotting devices, systems, and kits and methods for their use
    7.
    发明授权
    Electro-blotting devices, systems, and kits and methods for their use 有权
    电印设备,系统,套件及其使用方法

    公开(公告)号:US08394250B2

    公开(公告)日:2013-03-12

    申请号:US13371325

    申请日:2012-02-10

    IPC分类号: G01N27/447

    CPC分类号: G01N27/44739

    摘要: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.

    摘要翻译: 本发明提供了用于干印迹凝胶的干燥电印迹系统,其中该系统包括电印迹转移堆叠,其包含分析凝胶和印迹膜,阳极,与阳极和转移体之间的阳极并置的阳极凝胶基体的主体 叠层,阴极和阴极凝胶基体与阴极和转移叠层之间的阴极并置,其中阳极凝胶基质和阴极凝胶基质各自包含用于电泳转移的离子源。 干电印系统不使用在电印迹之前加入到系统中的任何液体缓冲液(例如当组装传输堆叠时)。 阳极,阴极或两者可以与电源分离,并且作为一次性电极组件的一部分提供,其还包括包括用于电泳转移的离子的凝胶基质体。

    ELECTRO-BLOTTING DEVICES, SYSTEMS, AND KITS AND METHODS FOR THEIR USE
    8.
    发明申请
    ELECTRO-BLOTTING DEVICES, SYSTEMS, AND KITS AND METHODS FOR THEIR USE 有权
    电动灭火装置,系统,工具及其使用方法

    公开(公告)号:US20120138465A1

    公开(公告)日:2012-06-07

    申请号:US13371325

    申请日:2012-02-10

    IPC分类号: G01N33/559

    CPC分类号: G01N27/44739

    摘要: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.

    摘要翻译: 本发明提供了用于干印迹凝胶的干燥电印迹系统,其中该系统包括电印迹转移堆叠,其包含分析凝胶和印迹膜,阳极,与阳极和转移体之间的阳极并置的阳极凝胶基体的主体 叠层,阴极和阴极凝胶基体与阴极和转移叠层之间的阴极并置,其中阳极凝胶基质和阴极凝胶基质各自包含用于电泳转移的离子源。 干电印系统不使用在电印迹之前加入到系统中的任何液体缓冲液(例如当组装传输堆叠时)。 阳极,阴极或两者可以与电源分离,并且作为一次性电极组件的一部分提供,其还包括包括用于电泳转移的离子的凝胶基质体。

    Electro-blotting devices, systems, and kits, and methods for their use
    9.
    发明申请
    Electro-blotting devices, systems, and kits, and methods for their use 有权
    电泳印刷装置,系统和试剂盒及其使用方法

    公开(公告)号:US20060272946A1

    公开(公告)日:2006-12-07

    申请号:US11357416

    申请日:2006-02-21

    IPC分类号: G01N27/00

    CPC分类号: G01N27/44739

    摘要: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.

    摘要翻译: 本发明提供了用于干印迹凝胶的干燥电印迹系统,其中该系统包括电印迹转移堆叠,其包含分析凝胶和印迹膜,阳极,与阳极和转移体之间的阳极并置的阳极凝胶基体的主体 叠层,阴极和阴极凝胶基体与阴极和转移叠层之间的阴极并置,其中阳极凝胶基质和阴极凝胶基质各自包含用于电泳转移的离子源。 干电印系统不使用在电印迹之前加入到系统中的任何液体缓冲液(例如当组装传输堆叠时)。 阳极,阴极或两者可以与电源分离,并且作为一次性电极组件的一部分提供,其还包括包括用于电泳转移的离子的凝胶基质体。

    Electro-blotting devices, systems, and kits, and methods for their use
    10.
    发明授权
    Electro-blotting devices, systems, and kits, and methods for their use 有权
    电泳印刷装置,系统和试剂盒及其使用方法

    公开(公告)号:US08173002B2

    公开(公告)日:2012-05-08

    申请号:US11357416

    申请日:2006-02-21

    IPC分类号: G01N27/447

    CPC分类号: G01N27/44739

    摘要: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.

    摘要翻译: 本发明提供了用于干印迹凝胶的干燥电印迹系统,其中该系统包括电印迹转移堆叠,其包含分析凝胶和印迹膜,阳极,与阳极和转移体之间的阳极并置的阳极凝胶基体的主体 叠层,阴极和阴极凝胶基体与阴极和转移叠层之间的阴极并置,其中阳极凝胶基质和阴极凝胶基质各自包含用于电泳转移的离子源。 干电印系统不使用在电印迹之前加入到系统中的任何液体缓冲液(例如当组装传输堆叠时)。 阳极,阴极或两者可以与电源分离,并且作为一次性电极组件的一部分提供,其还包括包括用于电泳转移的离子的凝胶基质体。