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    ELECTRO-BLOTTING DEVICES, SYSTEMS, AND KITS AND METHODS FOR THEIR USE
    1.
    发明申请
    ELECTRO-BLOTTING DEVICES, SYSTEMS, AND KITS AND METHODS FOR THEIR USE 审中-公开
    电动灭火装置，系统，工具及其使用方法

    公开(公告)号：US20090026079A1

    公开(公告)日：2009-01-29

    申请号：US12171880

    申请日：2008-07-11

    申请人： Ilana MARGALIT ,  Uri Yogev ,  Itay Sela ,  Yuri Katz ,  Adam Sartiel ,  Timothy Updyke

    发明人： Ilana MARGALIT ,  Uri Yogev ,  Itay Sela ,  Yuri Katz ,  Adam Sartiel ,  Timothy Updyke

    IPC分类号： B01D61/42

    CPC分类号： G01N27/44739

    摘要： The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.

    摘要翻译： 本发明提供了用于干印迹凝胶的干燥电印迹系统，其中该系统包括电印迹转移堆叠，其包含分析凝胶和印迹膜，阳极，与阳极和转移体之间的阳极并置的阳极凝胶基体的主体 叠层，阴极和阴极凝胶基体与阴极和转移叠层之间的阴极并置，其中阳极凝胶基质和阴极凝胶基质各自包含用于电泳转移的离子源。 干电印系统不使用在电印迹之前加入到系统中的任何液体缓冲液（例如当组装传输堆叠时）。 阳极，阴极或两者可以与电源分离，并且作为一次性电极组件的一部分提供，其还包括包括用于电泳转移的离子的凝胶基质体。

    Composite compositions for electrophoresis
    2.
    发明申请
    Composite compositions for electrophoresis 审中-公开
    电泳用复合组合物

    公开(公告)号：US20050121325A1

    公开(公告)日：2005-06-09

    申请号：US10946472

    申请日：2004-09-20

    申请人： Timothy Updyke ,  Ilana Margalit ,  Michael Thacker

    发明人： Timothy Updyke ,  Ilana Margalit ,  Michael Thacker

    IPC分类号： G01N27/447 ,  G01N27/453

    CPC分类号： G01N27/44747 ,  G01N27/44739

    摘要： The invention is drawn to composite agarose/acrylamide compositions and gels. In particular it relates to gels for the separation of molecules, particularly macromolecules such as proteins. The invention is also directed to the preparation of composite gels, the separation of molecules by techniques such as electrophoresis using such gels, and the transfer of proteins from such gels to a transfer membrane using an immunoblot transfer gel.

    摘要翻译： 本发明涉及复合琼脂糖/丙烯酰胺组合物和凝胶。 特别地，它涉及用于分离分子的凝胶，特别是大分子如蛋白质。 本发明还涉及复合凝胶的制备，通过诸如使用这种凝胶的电泳的技术分离分子，以及使用免疫印迹转移凝胶将蛋白从这种凝胶转移到转移膜。

    COMPOSITE COMPOSITIONS FOR ELECTROPHORESIS
    3.
    发明申请
    COMPOSITE COMPOSITIONS FOR ELECTROPHORESIS 审中-公开
    电泳用复合组合物

    公开(公告)号：US20130008790A1

    公开(公告)日：2013-01-10

    申请号：US13490387

    申请日：2012-06-06

    申请人： Timothy Updyke ,  Ilana Margalit ,  Michael Thacker

    发明人： Timothy Updyke ,  Ilana Margalit ,  Michael Thacker

    IPC分类号： B01D57/02 ,  C07K1/26

    CPC分类号： G01N27/44747 ,  G01N27/44739

    摘要： The invention is drawn to composite agarose/acrylamide compositions and gels. In particular it relates to gels for the separation of molecules, particularly macromolecules such as proteins. The invention is also directed to the preparation of composite gels, the separation of molecules by techniques such as electrophoresis using such gels, and the transfer of proteins from such gels to a transfer membrane using an immunoblot transfer gel.

    摘要翻译： 本发明涉及复合琼脂糖/丙烯酰胺组合物和凝胶。 特别地，它涉及用于分离分子的凝胶，特别是大分子如蛋白质。 本发明还涉及复合凝胶的制备，通过诸如使用这种凝胶的电泳的技术分离分子，以及使用免疫印迹转移凝胶将蛋白从这种凝胶转移到转移膜。

    Quantitative fluorescent protein standards
    6.
    发明授权
    Quantitative fluorescent protein standards 有权
    定量荧光蛋白标准品

    公开(公告)号：US08778685B2

    公开(公告)日：2014-07-15

    申请号：US13392493

    申请日：2010-08-25

    申请人： Thomas Diller ,  Timothy Updyke

    发明人： Thomas Diller ,  Timothy Updyke

    IPC分类号： G01N33/68 ,  G01N21/64 ,  G01N27/447

    CPC分类号： G01N33/6803 ,  B33Y80/00 ,  C07K1/26 ,  Y10T436/10 ,  Y10T436/105831

    摘要： The present invention provides dual labeled protein standards useful for the simultaneous determination of the molecular weight of a subject protein as well as the relative mass (i.e., amount) of the subject protein present in an electrophoresis lane. The invention is also directed to methods suitable for the preparation of such dual labeled protein standards and to methods of using such dual labeled proteins to simultaneously determine the molecular weight and the relative amount of a subject protein. Further embodiments are directed to the use dual labeled protein standards to make a more accurate determination of the amount of a protein present in an electrophoresis lane. Yet further embodiments are directed to kits containing the presently described dual protein standards. Dual labeled protein standards made and used in accordance with the embodiments set forth herein may be used to simultaneously determine the molecular weight and the relative amount of a subject protein in real time.

    摘要翻译： 本发明提供了用于同时测定受试蛋白的分子量以及存在于电泳泳道中的受试蛋白的相对质量（即，量）的双重标记蛋白质标准。 本发明还涉及适用于制备这种双重标记的蛋白质标准品的方法以及使用这种双标记蛋白质同时测定受试蛋白质的分子量和相对量的方法。 进一步的实施方案涉及使用双重标记的蛋白质标准物来更准确地测定电泳泳道中存在的蛋白质的量。 另外的实施方案涉及包含目前描述的双重蛋白质标准品的试剂盒。 根据本文所述的实施方案制备和使用的双重标记的蛋白质标准物可用于同时确定受试蛋白质的分子量和相对量。