公开(公告)号：US20090246851A1

公开(公告)日：2009-10-01

申请号：US12088152

申请日：2006-09-25

申请人： Hitoshi Sato ,  Yutaka Shoga ,  Shingo Koyama ,  Iyori Shirakura

发明人： Hitoshi Sato ,  Yutaka Shoga ,  Shingo Koyama ,  Iyori Shirakura

IPC分类号： C12N9/00

CPC分类号： C12N9/00 ,  C07K1/34 ,  C12N1/02

摘要： The present invention provides a process for producing an enzyme, which includes recovering an enzyme by microfiltering an enzyme-containing solution, having a cell density of 1% (v/v) or less and an enzyme concentration of 1% (w/v) or more in terms of the amount of proteins, with a cationic surfactant added in an amount of 0.01 to 1% (w/v) to the enzyme-containing solution.

摘要翻译： 本发明提供一种酶的制造方法，其包括通过微细化含有细胞密度为1％（v / v）以下，酶浓度为1％（w / v）的含酶溶液来回收酶， 或更多的蛋白质量，其中阳离子表面活性剂以0.01至1％（w / v）的量加入到含酶溶液中。