公开(公告)号：US07319014B2

公开(公告)日：2008-01-15

申请号：US10545905

申请日：2004-02-20

申请人： Ellen Guthrie ,  Theodore Davis ,  Jack Benner, II

发明人： Ellen Guthrie ,  Theodore Davis ,  Jack Benner, II

IPC分类号： C12Q1/42 ,  C12P21/06 ,  C12N9/16

CPC分类号： C12Q1/42 ,  C12N9/16

摘要： Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65° C. and efficiency of dephosphorylation activity at approximately neutral pH.

摘要翻译： 提供了一种碱性磷酸酶的组合物和用于过热表达和纯化耐热性的南极磷酸酶（TAP）的方法。 TAP的用途包括核酸，糖，肽和蛋白质的去磷酸化。 如本文所述的TAP相对于65℃下的热稳定性来自其它来源的磷酸酶的优点和在近似中性pH下的去磷酸化活性的效率。