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1.发明授权Overexpression, purification and characterization of a thermolabile phosphatase 有权过度表达，纯化和表征热不稳定磷酸酶公开(公告)号：US07319014B2
公开(公告)日：2008-01-15
申请号：US10545905
申请日：2004-02-20
申请人： Ellen Guthrie , Theodore Davis , Jack Benner, II
发明人： Ellen Guthrie , Theodore Davis , Jack Benner, II
摘要： Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65° C. and efficiency of dephosphorylation activity at approximately neutral pH.
摘要翻译： 提供了一种碱性磷酸酶的组合物和用于过热表达和纯化耐热性的南极磷酸酶（TAP）的方法。 TAP的用途包括核酸，糖，肽和蛋白质的去磷酸化。 如本文所述的TAP相对于65℃下的热稳定性来自其它来源的磷酸酶的优点和在近似中性pH下的去磷酸化活性的效率。
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2.发明申请Overexpression, purification and characterization of a thermoolabile phosphatase 有权过表达，纯化和表征热不稳定磷酸酶公开(公告)号：US20060252118A1
公开(公告)日：2006-11-09
申请号：US10545905
申请日：2004-02-20
申请人： Ellen Guthrie , Theodore Davis , Jack Benner
发明人： Ellen Guthrie , Theodore Davis , Jack Benner
摘要： Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65° C. and efficiency of dephosphorylation activity at approximately neutral pH.
摘要翻译： 提供了一种碱性磷酸酶的组合物和用于过热表达和纯化耐热性的南极磷酸酶（TAP）的方法。 TAP的用途包括核酸，糖，肽和蛋白质的去磷酸化。 如本文所述的TAP相对于65℃下的热稳定性来自其它来源的磷酸酶的优点和在近似中性pH下的去磷酸化活性的效率。
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公开(公告)号：US20110053215A1
公开(公告)日：2011-03-03
申请号：US12988344
申请日：2009-04-07
CPC分类号： C12Y302/01097 , C12N9/2402
摘要： Methods and compositions have been described that relate to a newly identified polypeptide family wherein each member has O-glycosidase activity and specified sequence characteristics. This family of enzymes can be used for example for cleaving O-linked glycans and for synthesis of neoglycopeptides or neoglycoproteins.
摘要翻译： 已经描述了与新鉴定的多肽家族相关的方法和组合物，其中每个成员具有O-糖苷酶活性和指定的序列特征。 这种酶家族可用于例如用于切割O-连接的聚糖并用于合成新糖肽或新糖蛋白。
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公开(公告)号：US08969034B2
公开(公告)日：2015-03-03
申请号：US12988344
申请日：2009-04-07
CPC分类号： C12Y302/01097 , C12N9/2402
摘要： Methods and compositions have been described that relate to a newly identified polypeptide family wherein each member has O-glycosidase activity and specified sequence characteristics. This family of enzymes can be used for example for cleaving O-linked glycans and for synthesis of neoglycopeptides or neoglycoproteins.
摘要翻译： 已经描述了与新鉴定的多肽家族相关的方法和组合物，其中每个成员具有O-糖苷酶活性和指定的序列特征。 这种酶家族可用于例如用于切割O-连接的聚糖并用于合成新糖肽或新糖蛋白。
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