公开(公告)号：US20130151244A1

公开(公告)日：2013-06-13

申请号：US13316430

申请日：2011-12-09

申请人： Wei-ge Chen ,  Zhengyou Zhang ,  Jaemo Yang

发明人： Wei-ge Chen ,  Zhengyou Zhang ,  Jaemo Yang

IPC分类号： G10L19/14

CPC分类号： G10L25/69

摘要： Speech quality estimation technique embodiments are described which generally involve estimating the human speech quality of an audio frame in a single-channel audio signal. A representation of a harmonic component of the frame is synthesized and used to compute a non-harmonic component of the frame. The synthesized harmonic component representation and the non-harmonic component are then used to compute a harmonic to non-harmonic ratio (HnHR). This HnHR is indicative of the quality of a user's speech and is designated as an estimate of the speech quality of the frame. In one implementation, the HnHR is used to establish a minimum speech quality threshold below which the quality of the user's speech is considered unacceptable. Feedback to the user is then provided based on whether the HnHR falls below the threshold.

摘要翻译： 描述了通常涉及在单声道音频信号中估计音频帧的人类语音质量的语音质量估计技术实施例。 合成帧的谐波分量的表示，并用于计算帧的非谐波分量。 然后使用合成谐波分量表示和非谐波分量来计算谐波到非谐波比（HnHR）。 该HnHR表示用户语音的质量，并且被指定为帧的语音质量的估计。 在一个实现中，HnHR用于建立最小语音质量阈值，低于该最低语音质量阈值，用户语音的质量被认为是不可接受的。 然后基于HnHR是否低于阈值来提供对用户的反馈。

公开(公告)号：US6020538A

公开(公告)日：2000-02-01

申请号：US71204

申请日：1998-05-01

申请人： Kyung-Hwan Han ,  Jaemo Yang

发明人： Kyung-Hwan Han ,  Jaemo Yang

IPC分类号： C12N15/31 ,  C12N15/82 ,  C12N15/54 ,  C12N15/65

CPC分类号： C12N15/8207

摘要： A method is disclosed for making genetically engineered orchid species using accelerated particle transformation. Meristem-tip tissue of orchid is cultured into a protocorm-like body (PLB). PLB is meristematic tissue and can be induced to regenerate into whole plant. In order to achieve efficient transformation it is necessary that relatively large numbers of meristematic cells are bombarded with the DNA-coated particles. After a particle-mediated transformation event, it is also important to stimulate continued meristemoid development before and during antibiotic selection and to reduce toxic effect of oxidized products of phenolics exuded after the wounding. It has been discovered that by using the techniques described in the present invention the meristematic tissue development, before and after the bombardment, can be stimulated while the toxic effect of oxidized phenolics can be reduced, resulting in successful transformation. The bombarded PLBs are multiplied and selected for the presence of gene products coded by the introduced genes. Shoot regeneration is then induced from the PLBs and transgenic seedlings are produced. Preferred plants for carrying out the present invention are the members of the genus Cymbidium.

摘要翻译： 公开了一种使用加速粒子转化来制造转基因植物兰花物种的方法。 兰花的分生组织培养成原生质体样体（PLB）。 PLB是分生组织，可诱导再生成全植株。 为了实现有效的转化，需要用DNA涂覆的颗粒轰击相对大量的分生细胞。 在粒子介导的转化事件之后，在抗生素选择之前和期间刺激持续的分生组织发育以及减少伤口后渗出的酚类氧化产物的毒性作用也是重要的。 已经发现，通过使用本发明中描述的技术，可以刺激在轰击之前和之后的分生组织发育，同时可以减少氧化酚类的毒性作用，导致成功的转化。 将被轰击的PLB乘以并选择由引入的基因编码的基因产物的存在。 然后从PLB诱导射击再生，并产生转基因幼苗。 用于实施本发明的优选植物是蕙兰属的成员。

公开(公告)号：US08731911B2

公开(公告)日：2014-05-20

申请号：US13316430

申请日：2011-12-09

申请人： Wei-ge Chen ,  Zhengyou Zhang ,  Jaemo Yang

发明人： Wei-ge Chen ,  Zhengyou Zhang ,  Jaemo Yang

IPC分类号： G10L21/00

CPC分类号： G10L25/69

摘要： Speech quality estimation technique embodiments are described which generally involve estimating the human speech quality of an audio frame in a single-channel audio signal. A representation of a harmonic component of the frame is synthesized and used to compute a non-harmonic component of the frame. The synthesized harmonic component representation and the non-harmonic component are then used to compute a harmonic to non-harmonic ratio (HnHR). This HnHR is indicative of the quality of a user's speech and is designated as an estimate of the speech quality of the frame. In one implementation, the HnHR is used to establish a minimum speech quality threshold below which the quality of the user's speech is considered unacceptable. Feedback to the user is then provided based on whether the HnHR falls below the threshold.

摘要翻译： 描述了通常涉及在单声道音频信号中估计音频帧的人类语音质量的语音质量估计技术实施例。 合成帧的谐波分量的表示，并用于计算帧的非谐波分量。 然后使用合成谐波分量表示和非谐波分量来计算谐波到非谐波比（HnHR）。 该HnHR表示用户语音的质量，并且被指定为帧的语音质量的估计。 在一个实现中，HnHR用于建立最小语音质量阈值，低于该最低语音质量阈值，用户语音的质量被认为是不可接受的。 然后基于HnHR是否低于阈值来提供对用户的反馈。