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公开(公告)号:US20110053777A1
公开(公告)日:2011-03-03
申请号:US12303888
申请日:2006-06-15
申请人: James H. Oard , Nengyi Zhang , Dearl E. Sanders
发明人: James H. Oard , Nengyi Zhang , Dearl E. Sanders
CPC分类号: C12N9/88 , C12N15/8274 , C12N15/8278
摘要: Nucleotide sequences are disclosed that may be used to impart herbicide resistance to green plants. The sources of novel herbicide resistance were originally isolated in mutant Coreopsis plants. Green plants transformed with these sequences are resistant to herbicides that normally inhibit acetolactate synthase (ALS), particularly imidazolinone and sulfonylurea herbicides.
摘要翻译: 公开了可用于赋予绿色植物抗除草剂抗性的核苷酸序列。 新型除草剂抗性的来源最初分离在突变科植物植物中。 用这些序列转化的绿色植物对通常抑制乙酰乳酸合酶(ALS),特别是咪唑啉酮和磺酰脲类除草剂的除草剂具有抗性。
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公开(公告)号:US06528701B1
公开(公告)日:2003-03-04
申请号:US09516277
申请日:2000-02-29
申请人: Jianlin Wang , James H. Oard
发明人: Jianlin Wang , James H. Oard
IPC分类号: C12N1509
CPC分类号: C12N15/8237 , C07K14/415
摘要: Four rice ubiquitin genes have been sequenced, including the promoter region. Among the four promoters, two belong to a class known as polyubiquitin genes and two to the class of ubiquitin fusion genes. The two polyubiquitin genes both comprise of 6 ubiquitin-monomers in the coding region. Promoters isolated from the polyubiquitin genes were shown to drive strong constitutive expression in transformed rice plants. Expression of the one polyubiquitin gene was induced by heat-shock treatment. The ubiquitin promoters can be used to drive expression of structural genes including but not limited to genes for herbicide resistance, resistance to pests, and tolerance to drought or other adverse environmental conditions.
摘要翻译: 已经对四个水稻泛素基因进行了测序,包括启动子区。 在四个启动子中,两个属于称为多聚遍在蛋白基因的类别,另外两个属于泛素融合基因。 两个多聚遍在蛋白基因在编码区中都含有6个泛素单体。 从多聚泛素基因中分离的启动子显示在转化的水稻植物中驱动强的组成型表达。 通过热休克处理诱导一种多聚遍在蛋白基因的表达。 泛素启动子可用于驱动结构基因的表达,包括但不限于除草剂抗性,抗病虫害性和耐干旱或其他不利环境条件的基因。
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