公开(公告)号：US5682884A

公开(公告)日：1997-11-04

申请号：US281131

申请日：1994-07-27

申请人： Hugh Allen Oliver Hill ,  Irving John Higgins ,  James Michael McCann ,  Graham Davis

发明人： Hugh Allen Oliver Hill ,  Irving John Higgins ,  James Michael McCann ,  Graham Davis

IPC分类号： A61B5/00 ,  C12Q1/00 ,  C12Q1/68 ,  G01N27/403 ,  G01N33/487 ,  G01N33/543 ,  A61B5/05

CPC分类号： A61B5/1486 ,  A61B5/14865 ,  A61B5/6848 ,  C12Q1/001 ,  C12Q1/004 ,  G01N27/4035 ,  G01N33/5438 ,  A61B2560/0418

摘要： A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a deposit of an enzyme capable of catalyzing a reaction involving the compound and (preferably) an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing the enzyme (and preferably the mediator) onto the substrate.

摘要翻译： 检测液体混合物中的化合物的当前代表的传感器系统具有适于可释放地附接到信号读出电路的双电极条。 条带包括适于可释放地附接到读出电路的细长支撑件（优选是平坦的）; 第一导体和第二导体各自沿着支撑件延伸并且包括用于连接到电路的装置。 定位成接触液体混合物和第一导体的活性电极包括能够催化涉及化合物和（优选）电子介体的反应的酶的沉积物，其能够在酶催化的反应和第一个 导体。 定位参考电极以接触混合物和第二导体。 该系统包括适于提供代表电流的电信号的电路。 例如通过将酶（优选介体）丝网印刷到基底上来制造双电极条。

公开(公告)号：US5727548A

公开(公告)日：1998-03-17

申请号：US469205

申请日：1995-06-06

申请人： Hugh Allen Oliver Hill ,  Irving John Higgins ,  James Michael McCann ,  Graham Davis

发明人： Hugh Allen Oliver Hill ,  Irving John Higgins ,  James Michael McCann ,  Graham Davis

IPC分类号： A61B5/00 ,  C12Q1/00 ,  C12Q1/68 ,  G01N27/403 ,  G01N33/487 ,  G01N33/543

CPC分类号： G01N33/5438 ,  A61B5/1486 ,  A61B5/6848 ,  C12Q1/001 ,  C12Q1/004 ,  C12Q1/6825 ,  G01N27/4035 ,  A61B2560/0418 ,  Y10T29/4914 ,  Y10T29/49153 ,  Y10T29/49169 ,  Y10T29/49224

摘要： A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a single layer deposit of an enzyme capable of catalyzing a reaction involving the compound, a conductive material, and an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing, an admixture of an enzyme a conductive material, and a mediator as a single layer onto the substrate.

摘要翻译： 检测液体混合物中的化合物的当前代表的传感器系统具有适于可释放地附接到信号读出电路的双电极条。 条带包括适于可释放地附接到读出电路的细长支撑件（优选是平坦的）; 第一导体和第二导体各自沿着支撑件延伸并且包括用于连接到电路的装置。 定位成接触液体混合物和第一导体的活性电极包括能够催化涉及化合物，导电材料和电子介体的反应的酶的单层沉积物，其能够在酶催化的 反应和第一个导体。 定位参考电极以接触混合物和第二导体。 该系统包括适于提供代表电流的电信号的电路。 双电极条例如通过丝网印刷，酶导电材料的混合物和作为单层的介体制成在基底上。

公开(公告)号：US5820551A

公开(公告)日：1998-10-13

申请号：US470352

申请日：1995-06-06

申请人： Hugh Allen Oliver Hill ,  Irving John Higgins ,  James Michael McCann ,  Graham Davis

发明人： Hugh Allen Oliver Hill ,  Irving John Higgins ,  James Michael McCann ,  Graham Davis

IPC分类号： A61B5/00 ,  C12Q1/00 ,  C12Q1/68 ,  G01N27/403 ,  G01N33/487 ,  G01N33/543 ,  A61B5/05

CPC分类号： A61B5/1486 ,  A61B5/14865 ,  A61B5/6848 ,  C12Q1/001 ,  C12Q1/004 ,  G01N27/4035 ,  G01N33/5438 ,  A61B2560/0418

摘要： A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a single layer deposit of an enzyme capable of catalyzing a reaction involving the compound, a conductive material and an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing an admixture of an enzyme, a conductive material, and a mediator as a single layer onto the substrate.

摘要翻译： 检测液体混合物中的化合物的当前代表的传感器系统具有适于可释放地附接到信号读出电路的双电极条。 条带包括适于可释放地附接到读出电路的细长支撑件（优选是平坦的）; 第一导体和第二导体各自沿着支撑件延伸并且包括用于连接到电路的装置。 定位成接触液体混合物和第一导体的活性电极包括能够催化涉及化合物，导电材料和电子介体的反应的酶的单层沉积物，其能够在酶催化反应之间传递电子 和第一个导体。 定位参考电极以接触混合物和第二导体。 该系统包括适于提供代表电流的电信号的电路。 双电极条例如通过将酶，导电材料和介体的混合物作为单层丝网印刷到基底上来制造。