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1.发明授权Method and composition for the detection and study of cellular activity or the like and means for applying such method 失效用于检测和研究细胞活性等的方法和组合物以及应用这种方法的方法
公开(公告)号：US4390620A
公开(公告)日：1983-06-28
申请号：US216911
申请日：1980-12-16
IPC分类号： C12Q1/00 , G01N27/416 , G01N33/487 , C12Q1/04 , C12Q1/06
CPC分类号： C12Q1/004 , Y10S435/808 , Y10S435/817 , Y10S436/904
摘要： The invention pertains to a method for the study of the behaviour or detection of cells, organites or cellular fractions, possibly present in a medium.It comprises providing said medium with the essential nutrients to promote the activity of the said cells, organites or cellular fractions, if any, as well as with an "electron transporter" such as lipoic acid which is liable of forming a redox system and of intervening in one of the metabolic routes involved by the above said activity, particularly growth of cells.The method of the invention then further comprises measuring and detecting a possible modification of the relative proportions of the oxidized and reduced form of the "electron transporter". The time required for such modification to possibly take place, as well as other parameters of the reaction can then be correlated to the presence in the medium concerned of a cellular activity or analogous.
摘要翻译： 本发明涉及研究可能存在于培养基中的细胞，组织细胞或细胞部分的行为或检测方法。 其包括向所述培养基提供必需营养物质以促进所述细胞，组织细胞或细胞级分（如果有的话）的活性，以及与可能形成氧化还原体系的“电子转运蛋白”如硫辛酸和介入 在上述活性涉及的代谢途径之一，特别是细胞生长。 本发明的方法还包括测量和检测“电子转运体”的氧化还原形式的相对比例的可能修饰。 可能发生这种改变所需的时间以及反应的其它参数可以与在细胞活性或类似物相关的培养基中的存在相关。
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