-
公开(公告)号:US06534271B2
公开(公告)日:2003-03-18
申请号:US09866513
申请日:2001-05-25
IPC分类号: C12Q168
CPC分类号: C12N15/10 , C12Q1/686 , Y10S977/902 , C12Q2565/543 , C12Q2565/515 , C12Q2565/125
摘要: The present invention provides for amplification methods for cloning and copying genetic material on surfaces as well as copying biological material insofar as, in a broader sense, it can be classified as a ligand-receptor system. The invention therefore relates in particular to a method for propagating ligands and receptors on at least two surfaces, comprising (a) immobilizing a first ligand on a first surface of a substantially solid phase; (b) adding a solution of receptors and binding complementary receptors to the first ligand; (c) transferring the receptor to a second surface and immobilizing the receptor at that location; (d) attaching an additional ligand to the immobilized receptor; and (e) transferring the additional ligand to the first surface and immobilizing it at that location, wherein the steps set forth above may be repeated,multiple times.
摘要翻译: 本发明提供了用于在表面上克隆和复制遗传物质以及复制生物材料的扩增方法,只要在更广泛的意义上它可以分类为配体 - 受体系统。 因此,本发明具体涉及用于在至少两个表面上传播配体和受体的方法,其包括(a)将第一配体固定在基本固相的第一表面上; (b)加入受体的溶液并将互补受体结合到第一配体上; (c)将受体转移到第二表面并将受体固定在该位置处; (d)将另外的配体连接到固定的受体上; 和(e)将另外的配体转移到第一表面并将其固定在该位置,其中上述步骤可以重复多次。