公开(公告)号：US20190249194A1

公开(公告)日：2019-08-15

申请号：US16397423

申请日：2019-04-29

申请人： George M. Church ,  Prashant G. Mali ,  Luhan Yang

发明人： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC分类号： C12N15/85 ,  C12N15/10 ,  C12N15/82 ,  C12N15/81 ,  C12N9/22 ,  C12N15/90 ,  C12N15/63 ,  C12N15/87 ,  C12N15/01

CPC分类号： C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

摘要： A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US20190249172A1

公开(公告)日：2019-08-15

申请号：US16077982

申请日：2017-02-16

申请人： The Regents of the University of California

发明人： Donald B. Kohn ,  Carmen Flores Bjurstroem

IPC分类号： C12N15/11 ,  C12N9/22 ,  C07K14/47

CPC分类号： C12N15/11 ,  C07K14/4747 ,  C12N9/22 ,  C12N15/10 ,  C12N15/102 ,  C12N2310/20 ,  C12N2800/80

摘要： The present disclosure provides methods for gene editing in stem cells. The methods generally involve modifying the stem cells by increasing the level of an apoptosis regulator in the stem cells; and introducing into the modified stem cells a genome editing composition.

公开(公告)号：US20190233889A1

公开(公告)日：2019-08-01

申请号：US16313706

申请日：2017-04-03

申请人： TOYOTA JIDOSHA KABUSHIKI KAISHA

发明人： Hiroyuki ENOKI ,  Yoshie TAKEUCHI

IPC分类号： C12Q1/6874 ,  C12N15/10

CPC分类号： C12Q1/6874 ,  C12N15/09 ,  C12N15/10 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q2600/16 ,  C40B40/06 ,  C40B50/06 ,  C12Q2521/101 ,  C12Q2525/179 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2527/143

摘要： A DNA library with excellent reproducibility is readily produced. A nucleic acid amplification reaction is conducted in a reaction solution containing genomic DNA and a random primer at a high concentration to obtain a DNA fragment by the nucleic acid amplification reaction using the genomic DNA as a template.

公开(公告)号：US20190151474A2

公开(公告)日：2019-05-23

申请号：US15509258

申请日：2015-09-08

申请人： Ron Weiss ,  Liliana Wroblewska ,  Velia Siciliano ,  Tasuku Kitada ,  Maria Hottelet Foley ,  Katie Bodner ,  Hirohide Saito ,  Kei Endo ,  Darrell J. Irvine ,  Tyler Wagner ,  Jacob Becraft

发明人： Ron Weiss ,  Liliana Wroblewska ,  Velia Siciliano ,  Tasuku Kitada ,  Maria Hottelet Foley ,  Katie Bodner ,  Hirohide Saito ,  Kei Endo ,  Darrell J. Irvine ,  Tyler Wagner ,  Jacob Becraft

IPC分类号： A61K48/00 ,  C12N15/85

CPC分类号： A61K48/0066 ,  C12N15/10 ,  C12N15/63 ,  C12N15/85 ,  C12N2840/102

摘要： Engineered synthetic RNA-based genetic circuits are provided that are regulated exclusively at the post-transcriptional level.

公开(公告)号：US20190117748A1

公开(公告)日：2019-04-25

申请号：US16092137

申请日：2017-04-06

申请人： BRANDEIS UNIVERSITY

发明人： Isaac J. KRAUSS ,  Joel Sebastian TEMME

IPC分类号： A61K39/00 ,  C12N15/117 ,  C07H15/22 ,  A61K39/39 ,  C12N15/115 ,  A61P31/18

CPC分类号： A61K39/0005 ,  A61K39/39 ,  A61K2039/51 ,  A61K2039/545 ,  A61P31/18 ,  C07H15/22 ,  C07H21/04 ,  C12N15/10 ,  C12N15/115 ,  C12N15/117 ,  C12N2310/13 ,  C12N2310/16 ,  C12N2310/351 ,  C12N2320/30

摘要： Disclosed are oligonucleotides that binds specifically to HIV neutralizing monoclonal antibody 2G12 with a K4 value lower than 20 nM, the oligonucleotide comprising a predicted structure, at 37° C., having exactly one stem-loop whereby the loop comprises the nucleotide sequence of AACCNACGGANAAA (SEQ ID NO: 1), where N is a modified nucleoside base, and the stem includes at least 3 nucleotide base-pairs and one of the nucleotides in the stem includes a modified nucleoside base, wherein the modified nucleoside base has the structure -B-L-A where A is a branched-chain Man9 oligosaccharide, L is a linker molecule, and B is independently a pyrimidine or pyridine base linked to the sugar-phosphate backbone of the oligonucleotide. Immunogenic conjugates that include the oligonucleotide, and pharmaceutical compositions that include the oligonucleotide or the immunogenic conjugate are also disclosed. Various method of using the oligonucleotides, immunogenic conjugates, and pharmaceutical compositions are also disclosed.

公开(公告)号：US20190078075A1

公开(公告)日：2019-03-14

申请号：US15776601

申请日：2016-11-11

申请人： Stratos Genomics, Inc.

发明人： Mark Stamatios Kokoris ,  Marc Prindle ,  Melud Nabavi ,  Craig Ostrander ,  Taylor Lehmann ,  Samantha Vellucci ,  Michael Kovarik ,  Jack Chase ,  Robert Busam ,  Miranda Lahman

IPC分类号： C12N15/10 ,  C07H21/04 ,  C12N9/12

CPC分类号： C12N15/10 ,  C07H21/04 ,  C12N9/1252 ,  C12Y207/07007

摘要： Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.

公开(公告)号：US20180312902A1

公开(公告)日：2018-11-01

申请号：US15770238

申请日：2016-10-21

申请人： UNIST(ULSAN NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY)

发明人： Yoon Kyoung CHO ,  Chi Ju KIM ,  Tae Hyeong KIM

IPC分类号： C12Q1/6806

CPC分类号： B01L3/00 ,  C12N15/10 ,  C12Q1/68 ,  G01N35/00 ,  G01N35/08

摘要： Provided is a nucleic acid purification device which is rotatably installed such that the entire processes of purifying a cell-free nucleic acid from a large amount of blood and a large amount of body fluid are integrated, the nucleic acid purification device including a disk in which a fluid is transferred by a centrifugal force, a supply portion installed in the disk and configured to supply a specimen and a reagent required for nucleic acid purification, an adsorption reaction chamber which is installed in the disk and is connected with the supply portion and in which an adsorption medium for adsorbing a nucleic acid is accommodated and the nucleic acid is adsorbed from the specimen, and a solution accommodating portion which is connected with an output side of an adsorption reaction portion along a centrifugal direction of the disk and in which a solution discharged through the adsorption reaction chamber by the centrifugal force is accommodated and is discharged to the outside.

公开(公告)号：US20180299457A1

公开(公告)日：2018-10-18

申请号：US16008777

申请日：2018-06-14

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Yao Zong NG ,  Pedro Alexis Baldera Aguayo ,  Virginia W. Cornish ,  Ehud Herbst

IPC分类号： G01N33/58 ,  G01N33/569 ,  C12N15/10

CPC分类号： G01N33/582 ,  C12N15/10 ,  C12N15/1086 ,  C12Q1/68 ,  C40B40/02 ,  G01N21/64 ,  G01N33/542 ,  G01N33/569 ,  G01N33/56944

摘要： The present disclosure provides a high-throughput fluorescence polarization microorganism screening assay for a metabolite produced by a microorganism. The metabolite is detected using competition with a fluorescent reporter for a receptor that binds both the metabolite and the reporter. The present disclosure further provides materials used in such an assay, a kit for such an assay, an isolated microorganism identified using such an assay, and culture conditions identified using such an assay.

公开(公告)号：US20180251788A1

公开(公告)日：2018-09-06

申请号：US15971863

申请日：2018-05-04

申请人： Caribou Biosciences, Inc.

发明人： Paul Daniel Donohoue ,  Andrew Paul May

IPC分类号： C12N15/90 ,  C12N15/113 ,  C12N9/22

CPC分类号： C12N15/902 ,  C12N9/22 ,  C12N15/10 ,  C12N15/113 ,  C12N2310/20 ,  C12N2310/51 ,  C12N2310/52

摘要： The present disclosure provides engineered polynucleotide sequences that form scaffolds and nucleoprotein complexes comprising such engineered polynucleotide sequences that form scaffolds and nucleic acid binding proteins. Nucleic acid sequences encoding the engineered polynucleotide sequences that form scaffolds, as well as expression cassettes, vectors and cells comprising such polynucleotide sequences, are described. A variety of methods for making and using the engineered polynucleotide sequences that form scaffolds are also disclosed.

公开(公告)号：US10053719B2

公开(公告)日：2018-08-21

申请号：US14765978

申请日：2014-03-13

申请人： Gen9, Inc.

发明人： Ishtiaq E. Saaem ,  Michael E. Hudson ,  Stephen F. Archer ,  Sophea Sann-Rorth ,  Ellen J. Sanchez ,  Joseph Jacobson

IPC分类号： C12P19/34 ,  C12N15/10 ,  C12N15/66

CPC分类号： C12P19/34 ,  C12N15/10 ,  C12N15/1031 ,  C12N15/66

摘要： Aspects of the invention relate to methods, compositions for synthesizing high fidelity oligonucleotides.