公开(公告)号：US20070049542A1

公开(公告)日：2007-03-01

申请号：US11432216

申请日：2006-05-10

申请人： Bruce Geller ,  Jesse Deere ,  Patrick Iversen ,  Dwight Weller

发明人： Bruce Geller ,  Jesse Deere ,  Patrick Iversen ,  Dwight Weller

IPC分类号： A61K48/00 ,  C07H21/02 ,  C07F9/6533

CPC分类号： C12N15/1137 ,  C07F9/65583 ,  C07F9/65613 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/3145 ,  C12N2310/3233

摘要： A method and antisense compound for inhibiting the growth of pathogenic bacterial cells are disclosed. The compound contains no more than 12 nucleotide bases and has a targeting nucleic acid sequence of no fewer than 10 bases in length that is complementary to a target sequence containing or within 10 bases, in a downstream direction, of the translational start codon of a bacterial mRNA that encodes a bacterial protein essential for bacterial replication. The compound binds to a target mRNA with a Tm of between 50° to 60° C. The relatively short antisense compounds are substantially more active than conventional antisense compounds having a targeting base sequence of 15 or more bases.

公开(公告)号：US20070021362A1

公开(公告)日：2007-01-25

申请号：US11173847

申请日：2005-07-01

申请人： Bruce Geller ,  Jesse Deere ,  Patrick Iversen

发明人： Bruce Geller ,  Jesse Deere ,  Patrick Iversen

IPC分类号： A61K48/00 ,  C07H21/02 ,  C07F9/6533

CPC分类号： C12N15/1137 ,  C07F9/65583 ,  C07F9/65613 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/3145 ,  C12N2310/3233

摘要： A method and antisense compound for inhibiting the growth of pathogenic bacterial cells are disclosed. The compound contains no more than 12 nucleotide bases and has a targeting nucleic acid sequence of no fewer than 10 bases in length that is complementary to a target sequence containing or within 10 bases, in a downstream direction, of the translational start codon of a bacterial mRNA that encodes a bacterial protein essential for bacterial replication. The compound binds to a target mRNA with a Tm of between 50° to 60° C. The relatively short antisense compounds are substantially more active than conventional antisense compounds having a targeting base sequence of 15 or more bases.

摘要翻译： 公开了一种用于抑制病原菌细胞生长的方法和反义化合物。 该化合物含有不超过12个核苷酸碱基，并且具有长度不小于10个碱基的靶向核酸序列，该靶向核酸序列与在细菌的翻译起始密码子的下游方向上含有或在10个碱基内的靶序列互补 编码细菌复制必需的细菌蛋白质的mRNA。 该化合物与50℃至60℃之间的T mRNA结合靶mRNA。相对较短的反义化合物比具有15个或更多个碱基的靶向碱基序列的常规反义化合物具有更多的活性 。