公开(公告)号：US07555938B2

公开(公告)日：2009-07-07

申请号：US11857615

申请日：2007-09-19

申请人： Igor Bargatin ,  Jessica L. Arlett ,  Michael L. Roukes ,  Inna Kozinsky ,  John Sequoyah Aldridge ,  Edward B. Myers

发明人： Igor Bargatin ,  Jessica L. Arlett ,  Michael L. Roukes ,  Inna Kozinsky ,  John Sequoyah Aldridge ,  Edward B. Myers

IPC分类号： G01R19/00 ,  H01L21/00

CPC分类号： G01N29/036 ,  G01N2291/0256 ,  Y10T29/41

摘要： Systems and methods for thermally actuating piezoresistive cantilevers are described. One embodiment includes a nanoelectromechanical resonator connected in at least one location to a substrate, an electrically conductive path formed on the resonator and a signal source connected to the electrically conductive path and configured to provide an oscillating actuation signal capable of exciting a resonant mode in the resonator.

摘要翻译： 描述了用于热致压压阻悬臂的系统和方法。 一个实施例包括在至少一个位置连接到衬底的纳米机电谐振器，形成在谐振器上的导电路径和连接到导电路径的信号源，并且被配置为提供能够激励谐振模式的振荡致动信号 谐振器。

公开(公告)号：US20100170797A1

公开(公告)日：2010-07-08

申请号：US12684357

申请日：2010-01-08

申请人： Jessica L. Arlett ,  Ji Hun Kim ,  Michael L. Roukes

发明人： Jessica L. Arlett ,  Ji Hun Kim ,  Michael L. Roukes

IPC分类号： B01D57/02

CPC分类号： B01D57/02 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0424 ,  B01L2400/046 ,  B03C5/005 ,  B03C5/026

摘要： A rapid and robust device and method for the capture and manipulation of single cells and beads in a microfluidic environment using positive dielectrophoresis (pDEP) is provided. The capture device uses a highly localized and non-uniform pDEP electric field gradient to allow for the simultaneous capture and manipulation of single cells and beads in standard cell growth media.

摘要翻译： 提供了使用正介电电泳（pDEP）在微流体环境中捕获和操纵单细胞和珠的快速且鲁棒的装置和方法。 捕获装置使用高度局部化和不均匀的pDEP电场梯度，以允许在标准细胞生长培养基中同时捕获和操纵单个细胞和珠粒。

公开(公告)号：US08476005B2

公开(公告)日：2013-07-02

申请号：US12364666

申请日：2009-02-03

申请人： Blake W. Axelrod ,  Michael L. Roukes ,  Jessica L. Arlett

发明人： Blake W. Axelrod ,  Michael L. Roukes ,  Jessica L. Arlett

IPC分类号： C12Q1/02 ,  C12P1/00 ,  C12M3/00 ,  C12N1/38

CPC分类号： C12Q1/02 ,  G01N33/502 ,  G01N2500/10

摘要： A method of screening one or more cells is described; the method includes: (i) providing one or more cells to a nanoelectromechanical system (NEMS) force sensor; (ii) applying at least one reagent to the one or more cells; and (iii) observing a response of the one or more cells to the reagent with the force sensor, thereby screening the one or more cells.

摘要翻译： 描述了筛选一个或多个细胞的方法; 该方法包括：（i）向纳米机电系统（NEMS）力传感器提供一个或多个单元; （ii）向所述一个或多个细胞施加至少一种试剂; 和（iii）用力传感器观察一个或多个细胞对试剂的反应，从而筛选该一个或多个细胞。

公开(公告)号：US20100041091A1

公开(公告)日：2010-02-18

申请号：US12364666

申请日：2009-02-03

申请人： Blake W. Axelrod ,  Michael L. Roukes ,  Jessica L. Arlett

发明人： Blake W. Axelrod ,  Michael L. Roukes ,  Jessica L. Arlett

IPC分类号： C12Q1/02

CPC分类号： C12Q1/02 ,  G01N33/502 ,  G01N2500/10

摘要： A method of screening one or more cells is described; the method includes: (i) providing one or more cells to a nanoelectromechanical system (NEMS) force sensor; (ii) applying at least one reagent to the one or more cells; and (iii) observing a response of the one or more cells to the reagent with the force sensor, thereby screening the one or more cells.

摘要翻译： 描述了筛选一个或多个细胞的方法; 该方法包括：（i）向纳米机电系统（NEMS）力传感器提供一个或多个单元; （ii）向所述一个或多个细胞施加至少一种试剂; 和（iii）用力传感器观察一个或多个细胞对试剂的反应，从而筛选该一个或多个细胞。

公开(公告)号：US07375321B2

公开(公告)日：2008-05-20

申请号：US10502551

申请日：2003-05-07

申请人： Michael L. Roukes ,  Scott E. Fraser ,  Jerry E. Solomon ,  Jessica L. Arlett ,  Michael C. Cross

发明人： Michael L. Roukes ,  Scott E. Fraser ,  Jerry E. Solomon ,  Jessica L. Arlett ,  Michael C. Cross

IPC分类号： G01N13/16

CPC分类号： G01N33/54373

摘要： A bioNEMS device comprises a piezoresistive cantilever having flexing legs of which attach the cantilever to a support and a biofunctionalized portion at the tip. A bias current applied to the legs is limited by a maximal acceptable temperature increase at the biofunctionalized tip. The length of the cantilever has a magnitude chosen to minimize background Johnson noise. A catalyzed receptor on the device binds to a ligand whose binding rate coefficient is enhanced. The catalyst lowers the receptor-ligand binding activation energy and is designed by forced evolution to preferentially bind with the ligand. A carrier signal is injected by a magnetic film disposed on the cantilever which is electromagnetically coupled to a source of the carrier signal. A plurality of NEMS fluidicly coupled transducers generate a plurality of output signals from which a collective output signal is derived, either by averaging or thresholding. The NEMS devices are disposed in microfluidic flow channels and fabricated in a membrane. A linking molecule is attached to the tip of the transducer and a fluffball attached to the linking molecule to increase damping.

摘要翻译： bioNEMS装置包括压阻悬臂，其具有将悬臂连接到支撑件上的弯曲腿和在尖端的生物功能化部分。 施加到腿上的偏置电流受生物功能化尖端的最大可接受的温度升高的限制。 悬臂的长度具有选择的最小化背景约翰逊噪声的大小。 器件上的催化受体与结合速率系数增强的配体结合。 催化剂降低受体 - 配体结合活化能，并通过强制进化来优先与配体结合而设计。 载体信号由布置在悬臂上的磁膜注入，该磁膜电磁耦合到载波信号源。 多个NEMS流体耦合的换能器产生多个输出信号，通过平均或阈值从该输出信号导出集体输出信号。 NEMS装置设置在微流体流动通道中并制成膜。 连接分子连接到换能器的尖端，并且连接到连接分子上的绒毛增加阻尼。