公开(公告)号：US07947821B2

公开(公告)日：2011-05-24

申请号：US12623527

申请日：2009-11-23

申请人： Changshen Liu ,  Jun Lin ,  Jiangchao Qian ,  Yuan Yuan

发明人： Changshen Liu ,  Jun Lin ,  Jiangchao Qian ,  Yuan Yuan

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12N15/63 ,  C12N15/85 ,  C12N15/86 ,  C12N1/20

CPC分类号： C07K14/51 ,  A61K38/00

摘要： The invention discloses an optimized DNA sequence of recombinant human bone morphogenetic protein-2 (rhBMP-2) based on the Escherichia coli expression system and a method for the preparation of the rhBMP-2. Specifically, the invention provides the optimal DNA sequences suitable for Escherichia coli expression system, the methods for efficient preparation of the rhBMP-2, and the related construction of the recombinant bacteria, the expression and purification technologies. Compared with the traditional hBMP-2 gene without optimizing, the rhBMP-2 expression level of the optimized gene in Escherichia coli is increased by 50%. Additionally, this invention also provides a method for preparation long chain rhBMP-2 with enhanced renaturation efficiency and yield of purification.

摘要翻译： 本发明公开了基于大肠杆菌表达系统的重组人骨形态发生蛋白-2（rhBMP-2）的优化DNA序列和rhBMP-2的制备方法。 具体而言，本发明提供了适用于大肠杆菌表达系统的最佳DNA序列，rhBMP-2的有效制备方法，以及重组细菌的相关构建，表达和纯化技术。 与未经优化的传统hBMP-2基因相比，大肠杆菌中优化基因的rhBMP-2表达水平提高了50％。 此外，本发明还提供了具有增强的复性效率和纯化产率的长链rhBMP-2的制备方法。

公开(公告)号：US20100190209A1

公开(公告)日：2010-07-29

申请号：US12623527

申请日：2009-11-23

申请人： Changsheng LIU ,  Jun LIN ,  Jiangchao QIAN ,  Yuan YUAN

发明人： Changsheng LIU ,  Jun LIN ,  Jiangchao QIAN ,  Yuan YUAN

IPC分类号： C12P21/02 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21

CPC分类号： C07K14/51 ,  A61K38/00

摘要： The invention discloses an optimized DNA sequence of recombinant human bone morphogenetic protein-2 (rhBMP-2) based on the Escherichia coli expression system and a method for the preparation of the rhBMP-2. Specifically, the invention provides the optimal DNA sequences suitable for Escherichia coli expression system, the methods for efficient preparation of the rhBMP-2, and the related construction of the recombinant bacteria, the expression and purification technologies. Compared with the traditional hBMP-2 gene without optimizing, the rhBMP-2 expression level of the optimized gene in Escherichia coli is increased by 50%. Additionally, this invention also provides a method for preparation long chain rhBMP-2 with enhanced renaturation efficiency and yield of purification.

摘要翻译： 本发明公开了基于大肠杆菌表达系统的重组人骨形态发生蛋白-2（rhBMP-2）的优化DNA序列和rhBMP-2的制备方法。 具体而言，本发明提供了适用于大肠杆菌表达系统的最佳DNA序列，rhBMP-2的有效制备方法，以及重组细菌的相关构建，表达和纯化技术。 与未经优化的传统hBMP-2基因相比，大肠杆菌中优化基因的rhBMP-2表达水平提高了50％。 此外，本发明还提供了具有增强的复性效率和纯化产率的长链rhBMP-2的制备方法。