公开(公告)号：US20100291696A1

公开(公告)日：2010-11-18

申请号：US12664465

申请日：2008-05-27

申请人： Joachim A. Stehr ,  Thomas A. Klar ,  Jochen Feldmann ,  Calin Hrelescu ,  Wolfgang Parak ,  Gunnar Raschke ,  Ralf Sperling ,  Michael Wunderlich ,  Konrad Kürzinger ,  Dieter Heindl ,  Alfons Nichtl

发明人： Joachim A. Stehr ,  Thomas A. Klar ,  Jochen Feldmann ,  Calin Hrelescu ,  Wolfgang Parak ,  Gunnar Raschke ,  Ralf Sperling ,  Michael Wunderlich ,  Konrad Kürzinger ,  Dieter Heindl ,  Alfons Nichtl

IPC分类号： G01N33/00

CPC分类号： C12Q1/6816 ,  Y10T436/143333 ,  C12Q2563/155 ,  C12Q2527/107

摘要： A process for detecting nucleic acids, having the following steps: providing at least one nanoparticle that is functionalised for the nucleic acid to be detected by means of at least one oligonucleotide that is bound to it and that is able to hybridize with at least one segment of a nucleic acid to be detected; bringing the functionalised nanoparticle into contact with a sample in which the nucleic acid is to be detected; and measuring a property that provides information about the degree of hybridization of the at least one oligonucleotide with the nucleic acid to be detected. In addition, the process includes the step of exciting the nanoparticles to generate heat, for example by means of a photothermal effect. The invention is suitable, in particular, for high-throughput DNA analysis.

摘要翻译： 一种用于检测核酸的方法，具有以下步骤：提供至少一种纳米颗粒，所述至少一种纳米颗粒通过与其结合的至少一种寡核苷酸为要检测的核酸而被官能化，并且能够与至少一个区段杂交 的待检测核酸; 使官能化的纳米颗粒与待检测核酸的样品接触; 并测量提供关于至少一种寡核苷酸与待检测核酸的杂交程度的信息的性质。 此外，该方法包括激发纳米颗粒以产生热量的步骤，例如通过光热效应。 本发明特别适用于高通量DNA分析。