公开(公告)号：US08553324B2

公开(公告)日：2013-10-08

申请号：US12673682

申请日：2008-08-06

申请人： Joerg Pacholik ,  Marco Hanft ,  Dieter Huhse

发明人： Joerg Pacholik ,  Marco Hanft ,  Dieter Huhse

IPC分类号： G02B21/00

CPC分类号： G02B21/006 ,  G02B21/0048 ,  G02B21/0072 ,  G02B21/241

摘要： The invention relates to a laser scanning microscope with a scanner and a microscope objective, and to a control method for such a microscope. In order to obtain sharp imaging of the sample in a laser scanning microscope, the distance between the microscope objective and the sample is usually varied for adjusting the focus position. However, relative movements between the objective and the sample can be problematic. In view of the costly special objective, internal focusing of the objective is a disadvantageous solution. An improved laser scanning microscope should make it possible to sharply image a sample with standard objectives without relative movement between the microscope objective and sample. According to the invention, a tube lens is provided which is displaceable along the optical axis, and the focus position is adjustable relative to a front optical element of the microscope objective by adjusting the tube lens.

摘要翻译： 本发明涉及具有扫描仪和显微镜物镜的激光扫描显微镜以及这种显微镜的控制方法。 为了在激光扫描显微镜中获得样品的清晰成像，显微镜物镜与样品之间的距离通常是变化的，用于调节聚焦位置。 然而，客观和样本之间的相对运动可能是有问题的。 鉴于昂贵的特殊目标，目标的内部重点是不利的解决方案。 改进的激光扫描显微镜应能使标准物体的样品锐利地成像，而不会在显微镜物镜和样品之间相对移动。 根据本发明，提供了一种可沿着光轴移动的管透镜，并且通过调节管透镜，聚焦位置可相对于显微镜物镜的前光学元件调节。

公开(公告)号：US20100214653A1

公开(公告)日：2010-08-26

申请号：US12673682

申请日：2008-08-06

申请人： Joerg Pacholik ,  Marco Hanft ,  Dieter Huhse

发明人： Joerg Pacholik ,  Marco Hanft ,  Dieter Huhse

IPC分类号： G02B21/02

CPC分类号： G02B21/006 ,  G02B21/0048 ,  G02B21/0072 ,  G02B21/241

摘要： The invention relates to a laser scanning microscope with a scanner and a microscope objective, and to a control method for such a microscope. In order to obtain sharp imaging of the sample in a laser scanning microscope, the distance between the microscope objective and the sample is usually varied for adjusting the focus position. However, relative movements between the objective and the sample can be problematic. In view of the costly special objective, internal focusing of the objective is a disadvantageous solution. An improved laser scanning microscope should make it possible to sharply image a sample with standard objectives without relative movement between the microscope objective and sample. According to the invention, a tube lens is provided which is displaceable along the optical axis, and the focus position is adjustable relative to a front optical element of the microscope objective by adjusting the tube lens.

摘要翻译： 本发明涉及具有扫描仪和显微镜物镜的激光扫描显微镜以及这种显微镜的控制方法。 为了在激光扫描显微镜中获得样品的清晰成像，显微镜物镜与样品之间的距离通常是变化的，用于调节聚焦位置。 然而，客观和样本之间的相对运动可能是有问题的。 鉴于昂贵的特殊目标，目标的内部重点是不利的解决方案。 改进的激光扫描显微镜应能使标准物体的样品锐利地成像，而不会在显微镜物镜和样品之间相对移动。 根据本发明，提供了一种可沿着光轴移动的管透镜，并且通过调节管透镜，聚焦位置可相对于显微镜物镜的前光学元件调节。

公开(公告)号：US20090303584A1

公开(公告)日：2009-12-10

申请号：US12375387

申请日：2007-07-24

申请人： Joerg Pacholik ,  Dieter Huhse ,  Thomas Paatzsch

发明人： Joerg Pacholik ,  Dieter Huhse ,  Thomas Paatzsch

IPC分类号： G02B21/06 ,  G02B27/10

CPC分类号： G02B21/002 ,  G01N21/6458

摘要： A method for laser scanning microscopy is characterized by the use of encapsulated fiber multiplexers from the telecommunications field for combining the beams of a plurality of lasers of different wavelengths and coupling them together into a laser scanning microscope and by corresponding beam combiners. Light-conducting guides to which different lasers can be coupled, preferably by light guides, are advantageously guided out of an encapsulated component.

摘要翻译： 激光扫描显微镜的方法的特征在于使用来自电信领域的封装光纤复用器，用于组合不同波长的多个激光束的光束并将它们耦合在一起成为激光扫描显微镜和相应的光束组合器。 优选地通过光导引入不同激光器的导光引导件有利地从封装的部件引出。