公开(公告)号：US20120301958A1

公开(公告)日：2012-11-29

申请号：US13481665

申请日：2012-05-25

申请人： Christian Kazanecki ,  David C. Colter ,  Johanna Schanz ,  Anke Hoppensack ,  Jan Hansmann ,  Heike Walles

发明人： Christian Kazanecki ,  David C. Colter ,  Johanna Schanz ,  Anke Hoppensack ,  Jan Hansmann ,  Heike Walles

IPC分类号： C12N5/071

CPC分类号： C12N5/0686 ,  C12N2503/00 ,  C12N2533/54 ,  C12N2533/92

摘要： This application invention discloses bioartificial proximal tubule device, constructed by preparing a decellularized biological matrix, seeding the biological matrix with mammalian kidney-derived cells and optionally mammalian endothelial cells. The device may then be cultured statically or matured using bioreactor culture to allow differentiation of the kidney cells into functioning proximal tubule cells. The device is capable of carrying out proximal tubule functions. The application also describes various methods of making the proximal tubule devices. The application also further describes methods of use of bioartificial proximal tubule devices for e.g. in vitro studies of tubule cell transport, toxicity effects of various compounds or pharmaceutical compound screening.

摘要翻译： 本发明公开了通过制备脱细胞生物基质构建的生物人造近端小管装置，用哺乳动物肾源细胞和任选的哺乳动物内皮细胞种植生物基质。 然后可以使用生物反应器培养静置或成熟培养该装置，以允许肾细胞分化成功能性的近端小管细胞。 该装置能够执行近端小管功能。 该应用还描述了制造近端小管装置的各种方法。 本申请还进一步描述了使用生物人造近端小管装置的方法。 小管细胞运输的体外研究，各种化合物的毒性作用或药物化合物筛选。