公开(公告)号：US06899915B2

公开(公告)日：2005-05-31

申请号：US09997734

申请日：2001-11-29

申请人： Pamela C. Yelick ,  John D. Bartlett ,  Joseph P. Vacanti ,  Bjorn R. Olsen ,  Phillip Stashenko

发明人： Pamela C. Yelick ,  John D. Bartlett ,  Joseph P. Vacanti ,  Bjorn R. Olsen ,  Phillip Stashenko

IPC分类号： A61K6/00 ,  A61K6/087 ,  A61L27/18 ,  A61L27/38 ,  A61C13/08

CPC分类号： A61L27/3804 ,  A61K6/087 ,  A61K6/10 ,  A61L27/18 ,  A61L27/3865 ,  C08L67/04 ,  C08L51/085

摘要： Tooth tissues include the pulp mesenchyme that forms the dentin and an epithelium that is responsible for enamel formation. Cells from these tissues were obtained from porcine third molars and were seeded onto a biodegradable scaffold composed of a polyglycolic acid—polylactic acid copolymer. Cell polymer constructs were then surgically implanted into the omentum of athymic nude rats so that the constructs would have a blood supply and these tissues were allowed to develop inside the rats. Infrequently, columnar epithelial cells were observed as a single layer on the outside of the dentin-like matrix similar to the actual arrangement of ameloblasts over dentin during early tooth development. Developing tooth tissues derived from such cell polymer constructs could eventually be surgically implanted into the gum of an edentulous recipient where the construct would receive a blood supply and develop to maturity, providing the recipient with a biological tooth replacement.

摘要翻译： 牙组织包括形成牙本质的牙髓间质和负责牙釉质形成的上皮。 来自这些组织的细胞从猪第三磨牙获得，并且接种到由聚乙醇酸 - 聚乳酸共聚物组成的可生物降解的支架上。 然后将细胞聚合物构建体手术植入无胸腺裸鼠的网膜中，使得构建体将具有血液供应，并且允许这些组织在大鼠内部发育。 不常见的是，柱状上皮细胞在牙质样基质的外侧被看作单层，类似于早期牙齿发育过程中釉质在牙本质上的实际布置。 来自这样的细胞聚合物构建体的牙齿组织的发育最终可以被手术植入无牙细胞受体的牙龈中，其中构建体将接受血液供应并发育成熟，从而为受体提供生物牙齿置换。