公开(公告)号：US4828440A

公开(公告)日：1989-05-09

申请号：US222698

申请日：1988-07-22

申请人： Robert L. Anderson ,  John E. Parks

发明人： Robert L. Anderson ,  John E. Parks

IPC分类号： F16B37/02 ,  F16B37/04

CPC分类号： F16B37/043 ,  F16B37/02 ,  Y10S411/97

摘要： A nut is comprised of a one piece stamping having a base wall with an integrally formed conical nut. A frame member has a bolt receiving first aperture in a first planar wall and a nut receiving second aperture in a second planar wall extending at a generally perpendicular angle with respect to the first planar wall. The stamping has integral stop tabs which engage with the frame member to limit the insertion of the stamping into the second aperture so that the conical nut is registered with the bolt receiving first aperture. First and second integral flexure arms flex inwardly upon insertion of the stamping through the second aperture and then engage with the inner surface of the frame member to retain the stamping against removal from the frame member. Integral flanges engage with the walls of the frame member defining the second aperture to cooperate with the stop tabs and the flexure arms to maintain the base wall in position registering the conical nut with the bolt receiving first aperture and to restrain the stamping against rotation relative to the frame member upon the threaded installation of a bolt into the conical nut.

摘要翻译： 螺母由单件冲压件构成，其具有带有整体形成的锥形螺母的底壁。 框架构件具有在第一平面壁中接收第一孔的螺栓和在相对于第一平面壁以大致垂直的角度延伸的第二平面壁中的第二孔的螺母。 冲压具有与框架构件接合以限制将冲压件插入第二孔中的整体止动片，使得锥形螺母与接收第一孔的螺栓对准。 第一和第二整体弯曲臂在冲压穿过第二孔插入时向内弯曲，然后与框架构件的内表面接合，以保持冲压而不从框架构件移除。 整体法兰与限定第二孔的框架构件的壁接合，以与止动接头和挠曲臂配合，以使基壁保持与螺栓接收第一孔对准的锥形螺母的位置，并且限制冲压相对于 框架构件在将螺栓螺纹安装到锥形螺母中时。

公开(公告)号：US06872569B2

公开(公告)日：2005-03-29

申请号：US10147576

申请日：2002-05-16

申请人： Dong Ryul Lee ,  Michael T. Kaproth ,  John E. Parks

发明人： Dong Ryul Lee ,  Michael T. Kaproth ,  John E. Parks

IPC分类号： C12N5/071 ,  C12N5/076 ,  C12N5/06 ,  A01N1/02 ,  C12N5/08

CPC分类号： C12N5/061 ,  C12N5/0697 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/31 ,  C12N2501/385 ,  C12N2501/392 ,  C12N2502/04 ,  C12N2502/246 ,  C12N2509/00

摘要： The present invention relates to a method of in vitro spermatogenesis involving Sertoli cells and diploid germ cells from a testis of a male mammal to yield differentiated haploid spermatids. The present invention also relates to spermatids produced by the method described above, where the spermatids are haploid. The present invention also involves a method of overcoming male infertility in mammals involving the use of the haploid round spermatids produced by the in vitro spermatogenesis method of the present invention. The present invention also relates to isolated haploid spermatids.

摘要翻译： 本发明涉及一种体外精子发生的方法，其涉及来自雄性哺乳动物的睾丸的Sertoli细胞和二倍体生殖细胞产生分化的单倍体精子细胞。 本发明还涉及通过上述方法制备的精子细胞，其中精子细胞是单倍体。 本发明还涉及克服哺乳动物中的男性不育症的方法，涉及使用通过本发明的体外精子发生方法产生的单倍体圆形精子细胞。 本发明还涉及分离的单倍体精子细胞。