公开(公告)号：US10280462B2

公开(公告)日：2019-05-07

申请号：US14312583

申请日：2014-06-23

申请人： Jacobus Johannes Maria Van Dongen ,  Anthonie Willem Langerak ,  Eduardus Maria Dominicus Schuuring ,  Jesus Fernando San Miguel ,  Ramon Garcia Sanz ,  Antonio Parreira ,  John Lewis Smith ,  Frances Louise Lavender ,  Gareth John Morgan ,  Paul Anthony Stuart Evans ,  Michael Kneba ,  Michael Hummel ,  Elizabeth Anne Macintyre ,  Christian Bastard ,  Frederic Bernard Louis Davi ,  Monika Brüggemann

发明人： Jacobus Johannes Maria Van Dongen ,  Anthonie Willem Langerak ,  Eduardus Maria Dominicus Schuuring ,  Jesus Fernando San Miguel ,  Ramon Garcia Sanz ,  Antonio Parreira ,  John Lewis Smith ,  Frances Louise Lavender ,  Gareth John Morgan ,  Paul Anthony Stuart Evans ,  Michael Kneba ,  Michael Hummel ,  Elizabeth Anne Macintyre ,  Christian Bastard ,  Frederic Bernard Louis Davi ,  Monika Brüggemann

IPC分类号： C12Q1/68 ,  C12Q1/6883 ,  C12Q1/6886

摘要： The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-Jκ IGK rearrangement, a VK/intron-Kde IGK rearrangement, a Vλ-Jλ IGL rearrangement, a Vβ-Jβ TCRB rearrangement, a Dβ-Jβ TCRB rearrangement, a Vγ-Jγ TCRG rearrangement, a Vδ-Jδ TCRD rearrangement, a Dδ-Dδ TCRD rearrangement, a Dδ-Jδ TCRD rearrangement, a Vδ-Dδ TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-IGH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.

公开(公告)号：US08859748B2

公开(公告)日：2014-10-14

申请号：US10531106

申请日：2003-10-13

申请人： Jacobus Johannes Maria Van Dongen ,  Anthonie Willem Langerak ,  Eduardus Maria Dominicus Schuuring ,  Jesus Fernando San Miquel ,  Ramon Garcia Sanz ,  Antonio Parreira ,  John Lewis Smith ,  Frances Louise Lavender ,  Gareth John Morgan ,  Paul Anthony Stuart Evans ,  Michael Kneba ,  Michael Hummel ,  Elizabeth Anne Macintyre ,  Christian Bastard

发明人： Jacobus Johannes Maria Van Dongen ,  Anthonie Willem Langerak ,  Eduardus Maria Dominicus Schuuring ,  Jesus Fernando San Miquel ,  Ramon Garcia Sanz ,  Antonio Parreira ,  John Lewis Smith ,  Frances Louise Lavender ,  Gareth John Morgan ,  Paul Anthony Stuart Evans ,  Michael Kneba ,  Michael Hummel ,  Elizabeth Anne Macintyre ,  Christian Bastard

IPC分类号： C07H21/04 ,  C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/16

摘要： The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-JK IGK rearrangement, a VK/intron-Kde IGK rearrangement, a Vλ-Jλ IGL rearrangement, a Vβ-Jβ TCRB rearrangement, a Dβ-Jβ TCRB rearrangement, a Vγ-Jγ TCRG rearrangement, a Vδ-Jδ TCRD rearrangement, a Dδ-Dδ TCRD rearrangement, a Dδ-Jδ TCRD rearrangement, a Vδ-Dδ TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-IGH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.

摘要翻译： 本发明涉及基于PCR的克隆性研究，用于淋巴增生性疾病的早期诊断。 提供了一组核酸扩增引物，其包含正向引物或其变体，以及能够扩增选自VH-JH IGH重排，DH- JH IGH重排，VK-JK IGK重排，VK /内含子-Kde IGK重排，Vλ-JλIGL重排，V＆Bgr; -J＆bgr; TCRB重排，D＆bgr; -J＆bgr; TCRB重排，Vγ-JγTCRG重排，Vδ-JδTCRD重排，Dδ-DδTCRD重排，Dδ-JδTCRD重排，Vδ-DδTCRD重排或选自t（11; 14）（ BCL1-IGH）和t（14; 18）（BCL2-IGH）。 引物可用于基于PCR的克隆性研究，用于早期诊断淋巴增生性疾病和检测最小残留病（MRD）。 还提供了包含本发明的至少一组引物的试剂盒。

公开(公告)号：US20150099646A1

公开(公告)日：2015-04-09

申请号：US14312583

申请日：2014-06-23

申请人： Jacobus Johannes Maria Van Dongen ,  Anthonie Willem Langerak ,  Eduardus Maria Dominicus Schuuring ,  Jesus Fernando San Miquel ,  Ramon Garcia Sanz ,  Antonio Parreira ,  John Lewis Smith ,  Frances Louise Lavender ,  Gareth John Morgan ,  Paul Anthony Stuart Evans ,  Michael Kneba ,  Michael Hummel ,  Elizabeth Anne Macintyre ,  Christian Bastard

发明人： Jacobus Johannes Maria Van Dongen ,  Anthonie Willem Langerak ,  Eduardus Maria Dominicus Schuuring ,  Jesus Fernando San Miquel ,  Ramon Garcia Sanz ,  Antonio Parreira ,  John Lewis Smith ,  Frances Louise Lavender ,  Gareth John Morgan ,  Paul Anthony Stuart Evans ,  Michael Kneba ,  Michael Hummel ,  Elizabeth Anne Macintyre ,  Christian Bastard

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/16

摘要： The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-JK IGK rearrangement, a VK/intron-Kde IGK rearrangement, a Vλ-Jλ IGL rearrangement, a Vβ-Jβ TCRB rearrangement, a Dβ-Jβ TCRB rearrangement, a Vγ-Jγ TCRG rearrangement, a Vδ-Jδ TCRD rearrangement, a Dδ-Dδ TCRD rearrangement, a Dδ-Jδ TCRD rearrangement, a Vδ-Dδ TCRD rearrangement, or a translocation selected from t(11;14) (BCL1-IGH) and t(14;18) (BCL2-IGH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.

摘要翻译： 本发明涉及基于PCR的克隆性研究，用于淋巴增生性疾病的早期诊断。 提供了一组核酸扩增引物，其包含正向引物或其变体，以及能够扩增选自VH-JH IGH重排，DH- JH IGH重排，VK-JK IGK重排，VK /内含子-Kde IGK重排，Vλ-JλIGL重排，V＆Bgr; -J＆bgr; TCRB重排，D＆bgr; -J＆bgr; TCRB重排，Vγ-JγTCRG重排，Vδ-JδTCRD重排，Dδ-DδTCRD重排，Dδ-JδTCRD重排，Vδ-DδTCRD重排或选自t（11; 14）（ BCL1-IGH）和t（14; 18）（BCL2-IGH）。 引物可用于基于PCR的克隆性研究，用于早期诊断淋巴增生性疾病和检测最小残留病（MRD）。 还提供了包含本发明的至少一组引物的试剂盒。

公开(公告)号：US20200172973A1

公开(公告)日：2020-06-04

申请号：US16404583

申请日：2019-05-06

申请人： Jacobus Johannes Maria van Dongen ,  Anthonie Willem Langerak ,  Eduardus Maria Dominicus Schuuring ,  Jesus Fernando San Miguel ,  Ramon Garcia Sanz ,  Antonio Parreira ,  John Lewis Smith ,  Frances Louise Lavender ,  Gareth John Morgan ,  Paul Anthony Stuart Evans ,  Michael Kneba ,  Michael Hummel ,  Elizabeth Anne Macintyre ,  Christian Bastard ,  Monika Brüggemann ,  Frederic Bernard Louis Davi

发明人： Jacobus Johannes Maria van Dongen ,  Anthonie Willem Langerak ,  Eduardus Maria Dominicus Schuuring ,  Jesus Fernando San Miguel ,  Ramon Garcia Sanz ,  Antonio Parreira ,  John Lewis Smith ,  Frances Louise Lavender ,  Gareth John Morgan ,  Paul Anthony Stuart Evans ,  Michael Kneba ,  Michael Hummel ,  Elizabeth Anne Macintyre ,  Christian Bastard ,  Monika Brüggemann ,  Frederic Bernard Louis Davi

IPC分类号： C12Q1/6883 ,  C12Q1/6886

摘要： The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-JK IGK rearrangement, a VK/intron-Kde IGK rearrangement, a Vλ-Jλ IGL rearrangement, a Vβ-Jβ TCRB rearrangement, a Dβ-Jβ TCRB rearrangement, a Vγ-Jγ TCRG rearrangement, a Vδ-Jδ TCRD rearrangement, a Dδ-Dδ TCRD rearrangement, a Dδ-Jδ TCRD rearrangement, a Vδ-Dδ TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-IGH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.