公开(公告)号：US20100137413A1

公开(公告)日：2010-06-03

申请号：US12521695

申请日：2007-02-16

申请人： Jordan Cummins ,  Victor Velculescu ,  Kenneth W. Kinzler ,  Bert Vogelstein

发明人： Jordan Cummins ,  Victor Velculescu ,  Kenneth W. Kinzler ,  Bert Vogelstein

IPC分类号： A61K31/711 ,  C07H21/02 ,  C12N5/071 ,  C12Q1/68 ,  G01N33/574 ,  C07H21/04 ,  A61K31/7105 ,  C12N5/09 ,  A61P35/00

CPC分类号： C12N15/113 ,  C12N2310/141 ,  C12N2320/11 ,  C12N2330/10 ,  C12N2510/00 ,  C12Q1/6886

摘要： MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we have developed an experimental approach called miRNA serial analysis of gene expression (miRAGE) and used it to perform the largest experimental analysis of human miRNAs to date. Sequence analysis of 273,966 small RNA tags from human colorectal cells allowed us to identify 200 known mature miRNAs, 133 novel miRNA candidates, and 112 previously uncharacterized miRNA* forms. To aid in the evaluation of candidate miRNAs, we disrupted the Dicer locus in three human colorectal cancer cell lines and examined known and novel miRNAs in these cells. The miRNAs are useful to diagnose and treat cancers.

摘要翻译： 微小RNA（miRNA）是一类在多细胞生物体中具有重要调节作用的小型非编码RNA。 公共miRNA数据库包含321个人类miRNA序列，其中234个已经通过实验验证。 为了探讨其他miRNA存在于人类基因组中的可能性，我们开发了一种称为miRNA序列分析基因表达（miRAGE）的实验方法，并将其用于迄今为止对人类miRNA进行最大的实验分析。 来自人结肠直肠细胞的273,966个小RNA标签的序列分析允许我们鉴定200个已知的成熟miRNA，133个新型miRNA候选物和112个先前未表征的miRNA *形式。 为了帮助评估候选miRNA，我们破坏了三种人结肠直肠癌细胞系中Dicer基因座，并检测了这些细胞中已知和新型的miRNA。 这些miRNA可用于诊断和治疗癌症。

公开(公告)号：US08586725B2

公开(公告)日：2013-11-19

申请号：US12521695

申请日：2007-02-16

申请人： Jordan Cummins ,  Victor Velculescu ,  Kenneth W. Kinzler ,  Bert Vogelstein

发明人： Jordan Cummins ,  Victor Velculescu ,  Kenneth W. Kinzler ,  Bert Vogelstein

IPC分类号： C07H21/02 ,  C07H21/04

CPC分类号： C12N15/113 ,  C12N2310/141 ,  C12N2320/11 ,  C12N2330/10 ,  C12N2510/00 ,  C12Q1/6886

摘要： MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we have developed an experimental approach called miRNA serial analysis of gene expression (miRAGE) and used it to perform the largest experimental analysis of human miRNAs to date. Sequence analysis of 273,966 small RNA tags from human colorectal cells allowed us to identify 200 known mature miRNAs, 133 novel miRNA candidates, and 112 previously uncharacterized miRNA* forms. To aid in the evaluation of candidate miRNAs, we disrupted the Dicer locus in three human colorectal cancer cell lines and examined known and novel miRNAs in these cells. The miRNAs are useful to diagnose and treat cancers.

摘要翻译： 微小RNA（miRNA）是一类在多细胞生物体中具有重要调节作用的小型非编码RNA。 公共miRNA数据库包含321个人类miRNA序列，其中234个已经通过实验验证。 为了探讨其他miRNA存在于人类基因组中的可能性，我们开发了一种称为miRNA序列分析基因表达（miRAGE）的实验方法，并将其用于迄今为止对人类miRNA进行最大的实验分析。 来自人结肠直肠细胞的273,966个小RNA标签的序列分析允许我们鉴定200个已知的成熟miRNA，133个新型miRNA候选物和112个先前未表征的miRNA *形式。 为了帮助评估候选miRNA，我们破坏了三种人结肠直肠癌细胞系中Dicer基因座，并检测了这些细胞中已知和新型的miRNA。 这些miRNA可用于诊断和治疗癌症。