公开(公告)号：US20090142809A1

公开(公告)日：2009-06-04

申请号：US12084721

申请日：2006-09-25

申请人： Uwe Horn ,  Armin Siering ,  Gundela Peschel ,  Christian Hertweck ,  Peter-Jurgen Muller ,  Bettina Bardl ,  Jorg-Hermann Ozegowski ,  Kerstin Herold

发明人： Uwe Horn ,  Armin Siering ,  Gundela Peschel ,  Christian Hertweck ,  Peter-Jurgen Muller ,  Bettina Bardl ,  Jorg-Hermann Ozegowski ,  Kerstin Herold

IPC分类号： C12P19/56

CPC分类号： C12P19/56

摘要： The invention relates to a method for cleaving cervimycin esters. An object of the invention to convert in a simple manner the cervimycin esters which are less active but formed in larger quantity into the highly active cervimycin K that occurs as a minor component in the preparation by fermentation, is achieved by preparing unesterified cervimycins from cervimycin esters with di- or monomethylated malonic acids, by ester cleavage effected by at least one esterolytic enzyme at temperatures of 20° C. to 75° C. and a pH of pH 5.0 to 10.0, preferably pH 6.0 to 9.0.

摘要翻译： 本发明涉及一种切割子宫颈霉素酯的方法。 本发明的一个目的是以简单的方式将活性较低但以大量形成的子宫颈素酯转化成通过发酵作为制剂中作为次要成分发生的高活性的宫颈霉素K，通过从宫颈霉素酯制备未酯化的cervimycins来实现 通过在20℃至75℃的温度下由至少一种酯水解酶进行的酯切割，pH为5.0至10.0，优选pH 6.0至9.0的pH进行酯化裂解，得到二 - 或单甲基丙二酸。