公开(公告)号：US06447770B1

公开(公告)日：2002-09-10

申请号：US09395861

申请日：1999-09-14

申请人： Jos M. Raaijmakers ,  David M. Weller ,  Linda S. Thomashow ,  R. James Cook

发明人： Jos M. Raaijmakers ,  David M. Weller ,  Linda S. Thomashow ,  R. James Cook

IPC分类号： A01N6300

CPC分类号： C12R1/38 ,  A01N63/00 ,  C12N15/52 ,  C12N15/78 ,  C12R1/39 ,  Y10S435/876

摘要： Fluorescent Pseudomonas spp. are described which are effective for the control of diseases caused by the soil-borne fungus, Gaeumannomyces graminis (Gg), such as take-all, in small grain crops or turf grass. The subject biocontrol strains have a unique genotype as shown by a characteristic banding pattern, and exhibit root-colonizing ability which is characterized by both higher population density on roots and extended colonizing activity compared to known Gg-suppressive strains. A further property is the ability of a strain to duplicate the level of biocontrol obtained naturally in a take-all decline soil. Methods for isolation and identification of the strains and their use to control diseases caused by Gg are provided. In particular, strains of P. fluorescens NRRL B-21806 and NRRL B-21807.

摘要翻译： 荧光假单胞菌 被描述为有效地控制由土壤传播的真菌，禾谷镰刀菌（Gg），如小麦作物或草坪草中所有的所有疾病引起的疾病。 主题生物防治菌株具有独特的基因型，如特征条带图案所示，并且表现出根瘤定殖能力，其特征在于与已知的Gg抑制菌株相比，根系的群体密度较高和延长的定居活动。 另一个属性是菌株复制在全部衰退土壤中自然获得的生物防治水平的能力。 提供了用于分离和鉴定菌株及其用于控制​​由Gg引起的疾病的方法。 特别是荧光假单胞菌NRRL B-21806和NRRL B-21807的菌株。

公开(公告)号：US06277625B1

公开(公告)日：2001-08-21

申请号：US08994035

申请日：1997-12-18

申请人： Zhengyu Huang ,  Linda S. Thomashow ,  Dmitri V. Mavrodi ,  Jos M. Raaijmakers ,  David M. Weller ,  R. James Cook

发明人： Zhengyu Huang ,  Linda S. Thomashow ,  Dmitri V. Mavrodi ,  Jos M. Raaijmakers ,  David M. Weller ,  R. James Cook

IPC分类号： C12N1578

CPC分类号： C12R1/38 ,  A01N63/00 ,  C12N15/52 ,  C12N15/78 ,  C12R1/39 ,  Y10S435/876

摘要： Transgenic fluorescent Pseudomonas spp. are described which have a biosynthetic locus which encodes for the production of the antibiotic phenazine-1-carboxylic acid stably introduced into the genome, have a locus which encodes for the production of the antibiotic 2,4-diacetylphloroglucinol, and are effective for control of diseases caused by the soil-borne fungus, Rhizoctonia. Strains are also described which control diseases caused by Gaeumannomyces graminis or Pythium, in addition to Rhizoctonia, or have the ability to control all three diseases.

摘要翻译： 转基因荧光假单胞菌 描述了具有编码产生稳定导入基因组中的抗生素吩嗪-1-羧酸的生物合成基因座，具有编码抗生素2,4-二乙酰基叶绿素的生产的场所，并且有效地控制 由土传真菌，丝核菌引起的疾病。 还描述了除了丝核菌之外还控制由禾谷镰刀菌（Gaeumannomyces graminis）或腐霉属（Pythium）引起的疾病或具有控制所有三种疾病的能力的菌株。