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公开(公告)号：US6051383A
公开(公告)日：2000-04-18
申请号：US251725
申请日：1999-02-17
IPC分类号： C07K14/21 , C12N15/52 , C12Q1/68 , C07K14/195
摘要： DNA sequences which function specifically in the synthesis of 2,4-diacetylphloroglucinol (Phl) are described. The sequences include phl genes which encode phl gene proteins and coding and regulatory sequences for production of Phl as well as sequences containing phl genes, which sequences have the capability of conferring or enhancing Phl biosynthetic capability in bacterial strains. The transformed strains are useful as biocontrol agents against fungal pathogens.
摘要翻译： 描述了在2,4-二乙酰基葡萄糖醇（Phl）的合成中特异性起作用的DNA序列。 序列包括编码ph1基因蛋白的ph1基因和用于产生Ph1的编码和调节序列以及含有ph1基因的序列，该序列具有赋予或增强细菌菌株中的Ph1生物合成能力的能力。 转化的菌株可用作抗真菌病原体的生物防治剂。
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公开(公告)号：US5955298A
公开(公告)日：1999-09-21
申请号：US494907
申请日：1995-06-26
摘要： DNA sequences which function specifically in the synthesis of 2,4-diacetylphloroglucinol (Phl) are described. The sequences include phl genes which encode phl gene proteins and coding and regulatory sequences for production of Phl as well as sequences containing phl genes, which sequences have the capability of conferring or enhancing Phl biosynthetic capability in bacterial strains. The transformed strains are useful as biocontrol agents against fungal pathogens.
摘要翻译： 描述了在2,4-二乙酰基葡萄糖醇（Phl）的合成中特异性起作用的DNA序列。 序列包括编码ph1基因蛋白的ph1基因和用于产生Ph1的编码和调节序列以及含有ph1基因的序列，该序列具有赋予或增强细菌菌株中的Ph1生物合成能力的能力。 转化的菌株可用作抗真菌病原体的生物防治剂。
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公开(公告)号：US06447770B1
公开(公告)日：2002-09-10
申请号：US09395861
申请日：1999-09-14
IPC分类号： A01N6300
摘要： Fluorescent Pseudomonas spp. are described which are effective for the control of diseases caused by the soil-borne fungus, Gaeumannomyces graminis (Gg), such as take-all, in small grain crops or turf grass. The subject biocontrol strains have a unique genotype as shown by a characteristic banding pattern, and exhibit root-colonizing ability which is characterized by both higher population density on roots and extended colonizing activity compared to known Gg-suppressive strains. A further property is the ability of a strain to duplicate the level of biocontrol obtained naturally in a take-all decline soil. Methods for isolation and identification of the strains and their use to control diseases caused by Gg are provided. In particular, strains of P. fluorescens NRRL B-21806 and NRRL B-21807.
摘要翻译： 荧光假单胞菌 被描述为有效地控制由土壤传播的真菌，禾谷镰刀菌（Gg），如小麦作物或草坪草中所有的所有疾病引起的疾病。 主题生物防治菌株具有独特的基因型，如特征条带图案所示，并且表现出根瘤定殖能力，其特征在于与已知的Gg抑制菌株相比，根系的群体密度较高和延长的定居活动。 另一个属性是菌株复制在全部衰退土壤中自然获得的生物防治水平的能力。 提供了用于分离和鉴定菌株及其用于控制由Gg引起的疾病的方法。 特别是荧光假单胞菌NRRL B-21806和NRRL B-21807的菌株。
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4.发明授权公开(公告)号：US06277625B1
公开(公告)日：2001-08-21
申请号：US08994035
申请日：1997-12-18
申请人： Zhengyu Huang , Linda S. Thomashow , Dmitri V. Mavrodi , Jos M. Raaijmakers , David M. Weller , R. James Cook
发明人： Zhengyu Huang , Linda S. Thomashow , Dmitri V. Mavrodi , Jos M. Raaijmakers , David M. Weller , R. James Cook
IPC分类号： C12N1578
摘要： Transgenic fluorescent Pseudomonas spp. are described which have a biosynthetic locus which encodes for the production of the antibiotic phenazine-1-carboxylic acid stably introduced into the genome, have a locus which encodes for the production of the antibiotic 2,4-diacetylphloroglucinol, and are effective for control of diseases caused by the soil-borne fungus, Rhizoctonia. Strains are also described which control diseases caused by Gaeumannomyces graminis or Pythium, in addition to Rhizoctonia, or have the ability to control all three diseases.
摘要翻译： 转基因荧光假单胞菌 描述了具有编码产生稳定导入基因组中的抗生素吩嗪-1-羧酸的生物合成基因座，具有编码抗生素2,4-二乙酰基叶绿素的生产的场所，并且有效地控制 由土传真菌，丝核菌引起的疾病。 还描述了除了丝核菌之外还控制由禾谷镰刀菌（Gaeumannomyces graminis）或腐霉属（Pythium）引起的疾病或具有控制所有三种疾病的能力的菌株。
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5.发明授权Method for screening bacteria and application thereof for field control of diseases caused by Gaeumannomyces graminis 失效筛选细菌的方法及其应用于禾谷镰刀菌引起的疾病的田间防治
公开(公告)号：US4456684A
公开(公告)日：1984-06-26
申请号：US415778
申请日：1982-09-08
CPC分类号： C12R1/38 , A01N63/00 , C12N1/20 , Y10S435/876 , Y10S435/911
摘要： A method for screening bacteria to select strains which will suppress diseases caused by the fungus Gaeumannomyces graminis (Gg) under field conditions and a method for applying field-suppressive bacteria to suppress Gg in a commercial setting. Four fluorescens Pseudomonas strains are disclosed which are effective in suppressing take-all in wheat.
摘要翻译： 一种筛选细菌以选择在野外条件下抑制由真菌Gaeumannomyces graminis（Gg）引起的疾病的菌株的方法，以及在商业环境中应用场抑制细菌抑制Gg的方法。 公开了四种荧光假单胞菌菌株，其有效抑制小麦中的全部产生。
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6.发明授权Methods and compositions for the simultaneous control of the root diseases caused by gaeumannomyces graminis, rhizoctonia, and pythium 失效用于同时控制禾谷镰刀菌，丝核菌和pythium的根系疾病的方法和组合物
公开(公告)号：US5972689A
公开(公告)日：1999-10-26
申请号：US788604
申请日：1997-01-24
摘要： Methods and products useful to simultaneously control the root diseases caused by Gaeumannomyces graminis, Rhizoctonia, and Pythium in crops susceptible to these root diseases are described. These include a method to identify seed-treatment products for potential to simultaneously control the three kinds of root diseases; Bacillus strains having activity against the three kinds of root diseases and methods of obtaining the same, and compositions comprising a fungicide and a Bacillus strain having activity against the three kinds of root diseases, which compositions are useful for field control of the combination of the three kinds of root diseases.
摘要翻译： 描述了用于同时控制由禾谷镰刀菌，丝核菌和腐霉属引起的对这些根系疾病易感的作物的根系疾病的方法和产品。 这些包括识别可能同时控制三种根系疾病的种子处理产品的方法; 具有针对三种根系疾病的活性的芽孢杆菌菌株及其获得方法，以及包含杀真菌剂和具有抗三种根系疾病的活性的芽孢杆菌菌株的组合物，该组合物可用于田间控制三种 各种根系疾病
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7.发明授权Method for screening bacteria and application thereof for field control of Pythium spp. on small grain crops 失效筛选细菌的方法及其应用于腐霉菌的田间防治。 对小粮食作物
公开(公告)号：US4647533A
公开(公告)日：1987-03-03
申请号：US650739
申请日：1984-09-14
申请人： David M. Weller , R. James Cook , J. Ole Becker
发明人： David M. Weller , R. James Cook , J. Ole Becker
CPC分类号： C12R1/38 , A01N63/00 , C12Q1/02 , Y10S435/876 , Y10S435/877
摘要： A method for screening bacteria to select strains which will suppress Pythium spp. in small grain crops under field conditions and a method for applying field-suppressive bacteria to suppress Pythium spp. in a commercial setting are described. Four Pseudomonas strains which passed the screen test are disclosed.
摘要翻译： 筛选细菌以选择将抑制腐霉属的菌株的方法。 在田间条件下的小粮食作物和一种应用场抑制细菌抑制腐霉属的方法。 在商业设置中进行描述。 披露了通过筛选试验的四种假单胞菌菌株。
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