公开(公告)号：US20100080824A1

公开(公告)日：2010-04-01

申请号：US11808408

申请日：2007-06-08

申请人： Joseph S.M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls ,  Frederick C. Leung

发明人： Joseph S.M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls ,  Frederick C. Leung

IPC分类号： A61K39/12 ,  A61P31/12 ,  C07H19/00 ,  C12N5/06 ,  C12N7/00 ,  C12N7/04 ,  C12Q1/70

CPC分类号： A61K39/215 ,  A61K39/12 ,  A61K2039/6031 ,  C12N7/00 ,  C12N2770/20021 ,  C12N2770/20034 ,  C12Q1/6844 ,  C12Q1/701

摘要： The present invention relates to an isolated novel virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). The hSARS virus is identified to be morphologically and phylogenetically similar to known member of Coronaviridae. The present invention provides the complete genomic sequence of the hSARS virus. Furthermore, the invention provides the nucleic acids and peptides encoded by and/or derived from the hSARS virus and their use in diagnostic methods and therapeutic methods, including vaccines. In addition, the invention provides chimeric or recombinant viruses encoded by said nucleotide sequences and antibodies immunospecific to the polypeptides encoded by the nucleotide sequences.

摘要翻译： 本发明涉及在人类中引起严重急性呼吸综合征（SARS）的分离的新型病毒（“hSARS病毒”）。 鉴定hSARS病毒在形态和系统发育上与已知的冠状病毒科成员相似。 本发明提供了hSARS病毒的完整基因组序列。 此外，本发明提供由hSARS病毒编码和/或衍生自hSARS病毒的核酸和肽，以及它们在诊断方法和治疗方法（包括疫苗）中的用途。 此外，本发明提供由所述核苷酸序列编码的嵌合或重组病毒和对由核苷酸序列编码的多肽免疫特异性的抗体。

公开(公告)号：US20100285457A1

公开(公告)日：2010-11-11

申请号：US12483301

申请日：2009-06-12

申请人： Joseph S.M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls ,  Frederick C. Leung

发明人： Joseph S.M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls ,  Frederick C. Leung

IPC分类号： C12Q1/68

CPC分类号： C12Q1/701 ,  C12Q1/6844 ,  Y02A50/451

摘要： The present invention relates to a high-throughput diagnostic assay for the virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). In particular, the invention relates to a high-throughput reverse transcription-PCR diagnostic test for SARS associated coronavirus (SARS-CoV). The present assay is a rapid, reliable assay which can be used for diagnosis and monitoring the spread of SARS and is based on the nucleotide sequences of the N (nucleocapsid)-gene of the hSARS virus. The present method eliminates false negative results and provides increased sensitivity for the assay. The invention also discloses the S (spike)-gene of the hSARS virus. The invention further relates to the deduced amino acid sequences of the N-gene and S-gene products of the hSARS virus and to the use of the N-gene and S-gene products in diagnostic methods. The invention further encompasses diagnostic assays and kits comprising antibodies generated against the N-gene or S-gene product.

摘要翻译： 本发明涉及用于引起人类严重急性呼吸综合征（SARS）的病毒（“hSARS病毒”）的高通量诊断测定。 特别地，本发明涉及SARS相关冠状病毒（SARS-CoV）的高通量逆转录PCR诊断试验。 本试验是一种快速，可靠的测定法，可用于诊断和监测SARS的扩散，并且基于hSARS病毒的N（核衣壳）基因的核苷酸序列。 本方法消除假阴性结果并提供增加的检测灵敏度。 本发明还公开了hSARS病毒的S（尖峰） - 基因。 本发明还涉及hSARS病毒的N-基因和S-基因产物的推断氨基酸序列以及在诊断方法中使用N-基因和S-基因产物。 本发明还包括诊断测定和试剂盒，其包含针对N-基因或S-基因产物产生的抗体。

公开(公告)号：US20100184019A1

公开(公告)日：2010-07-22

申请号：US12703418

申请日：2010-02-10

申请人： Joseph S.M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls ,  Frederick C. Leung

发明人： Joseph S.M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls ,  Frederick C. Leung

IPC分类号： C12Q1/70 ,  C07H21/02 ,  C07H21/04 ,  C12N7/00 ,  C12Q1/68

CPC分类号： A61K39/215 ,  A61K39/12 ,  A61K2039/6031 ,  C12N7/00 ,  C12N2770/20021 ,  C12N2770/20034 ,  C12Q1/6844 ,  C12Q1/701

摘要： The present invention relates to an isolated novel virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). The hSARS virus is identified to be morphologically and phylogenetically similar to known member of Coronaviridae. The present invention provides the complete genomic sequence of the hSARS virus. Furthermore, the invention provides the nucleic acids and peptides encoded by and/or derived from the hSARS virus and their use in diagnostic methods and therapeutic methods, including vaccines. In addition, the invention provides chimeric or recombinant viruses encoded by said nucleotide sequences and antibodies immunospecific to the polypeptides encoded by the nucleotide sequences.

摘要翻译： 本发明涉及在人类中引起严重急性呼吸综合征（SARS）的分离的新型病毒（“hSARS病毒”）。 鉴定hSARS病毒在形态和系统发育上与已知的冠状病毒科成员相似。 本发明提供了hSARS病毒的完整基因组序列。 此外，本发明提供由hSARS病毒编码和/或衍生自hSARS病毒的核酸和肽，以及它们在诊断方法和治疗方法（包括疫苗）中的用途。 此外，本发明提供由所述核苷酸序列编码的嵌合或重组病毒和对由核苷酸序列编码的多肽免疫特异性的抗体。

公开(公告)号：US20080069838A1

公开(公告)日：2008-03-20

申请号：US10808121

申请日：2004-03-24

申请人： Joseph S.M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls ,  Frederick C. Leung

发明人： Joseph S.M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls ,  Frederick C. Leung

IPC分类号： A61K39/215 ,  C12N7/00

CPC分类号： A61K39/215 ,  A61K39/12 ,  A61K2039/6031 ,  C12N7/00 ,  C12N2770/20021 ,  C12N2770/20034 ,  C12Q1/6844 ,  C12Q1/701

摘要： The present invention relates to an isolated novel virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). The hSARS virus is identified to be morphologically and phylogenetically similar to known member of Coronaviridae. The present invention provides the complete genomic sequence of the hSARS virus. Furthermore, the invention provides the nucleic acids and peptides encoded by and/or derived from the hSARS virus and their use in diagnostic methods and therapeutic methods, including vaccines. In addition, the invention provides chimeric or recombinant viruses encoded by said nucleotide sequences and antibodies immunospecific to the polypeptides encoded by the nucleotide sequences.

摘要翻译： 本发明涉及在人类中引起严重急性呼吸综合征（SARS）的分离的新型病毒（“hSARS病毒”）。 鉴定hSARS病毒在形态和系统发育上与已知的冠状病毒科成员相似。 本发明提供了hSARS病毒的完整基因组序列。 此外，本发明提供由hSARS病毒编码和/或衍生自hSARS病毒的核酸和肽，以及它们在诊断方法和治疗方法（包括疫苗）中的用途。 此外，本发明提供由所述核苷酸序列编码的嵌合或重组病毒和对由核苷酸序列编码的多肽免疫特异性的抗体。