公开(公告)号：US5340926A

公开(公告)日：1994-08-23

申请号：US102697

申请日：1993-08-05

申请人： Peter A. Lowe ,  Fiona A. O. Marston ,  Sarojani Angal ,  Joyce A. Schoemaker

发明人： Peter A. Lowe ,  Fiona A. O. Marston ,  Sarojani Angal ,  Joyce A. Schoemaker

IPC分类号： C07K1/113 ,  C12N9/64 ,  C07K3/12 ,  C07K15/06

CPC分类号： C12N9/6481 ,  C07K1/1136

摘要： In a process for the production of a soluble native protein, such as immunoglobulin or methionine-prochymosin, in which an insoluble form of the protein is produced by a host organism transformed with a vector including a gene coding for the protein, the insoluble form of the protein is reversibly denatured in an alkaline aqueous solution at a pH selected to promote dissociation of a group or groups of the protein involved in maintaining the conformation of the protein, and the protein is subsequently allowed to renature by reducing the pH of the solution below a pH effective to denature the protein to produce the soluble native form of the protein. The pH of the alkaline aqueous is suitably in the range 9.0 to 11.5.

摘要翻译： 在生产可变天然蛋白质例如免疫球蛋白或甲硫氨酸凝乳酶原的方法中，其中蛋白质的不溶形式由宿主生物体转化，宿主生物体用包含编码蛋白质的基因的载体转化， 该蛋白质在选择用于促进参与维持蛋白质构象的一组或多组蛋白质的解离的pH下在碱性水溶液中可逆地变性，并且随后通过降低下述溶液的pH来使蛋白质复性 有效地使蛋白质变性以产生可溶性天然形式的蛋白质的pH。 碱性水溶液的pH适宜为9.0〜11.5。