公开(公告)号：US4132767A

公开(公告)日：1979-01-02

申请号：US783460

申请日：1977-03-31

申请人： Jun-ichi Tohmatsu ,  Tomiaki Morimoto ,  Emiko Kishi

发明人： Jun-ichi Tohmatsu ,  Tomiaki Morimoto ,  Emiko Kishi

IPC分类号： C07K16/18 ,  G01N33/531 ,  A61K43/00 ,  G01N33/16

CPC分类号： C07K16/18 ,  G01N33/531 ,  Y10S436/811 ,  Y10S436/82 ,  Y10S530/806 ,  Y10S530/83

摘要： Process for the preparation of a novel .alpha.-L antibody comprising immunizing an animal other than a human with the .alpha.-L antigen or .alpha.-L antigen/.alpha.-L antibody complex, and collecting antiserum from the animal; a method for the purification of .alpha.-L antigen comprising subjecting the material containing the .alpha.-L antigen to the density gradient centrifugation, and collecting a fraction having a buoyant density less than 1.1 g/ml; a reagent comprising .alpha.-L antigen; and a reagent comprising .alpha.-L antibody for detection of the .alpha.-L antigen and the .alpha.-L antibody, such detection being useful for the diagnosis of liver diseases and examination of the blood for blood transfusion.

摘要翻译： 用于制备新的α-L抗体的方法，包括用α-L抗原或α-L抗原/α-L抗体复合物免疫除人以外的动物，以及从动物收集抗血清; 用于纯化α-L抗原的方法，包括使含有α-L抗原的物质进行密度梯度离心，并收集浮力密度小于1.1g / ml的级分; 包含α-L抗原的试剂; 和包含用于检测α-L抗原和α-L抗体的α-L抗体的试剂，所述检测可用于诊断肝脏疾病和血液检查用于输血。