公开(公告)号：US4743544A

公开(公告)日：1988-05-10

申请号：US609530

申请日：1984-05-14

申请人： Yuzaburo Namba ,  Tohru Naraki ,  Takashi Sawada ,  Toyohiro Kitamura ,  Minoru Tohda ,  Tomiaki Morimoto

发明人： Yuzaburo Namba ,  Tohru Naraki ,  Takashi Sawada ,  Toyohiro Kitamura ,  Minoru Tohda ,  Tomiaki Morimoto

IPC分类号： G01N33/543 ,  B01L3/00 ,  G01N33/53 ,  G01N33/566

CPC分类号： B01L3/50851 ,  G01N33/53

摘要： Improved method for the measurement of substance comprising simultaneously measuring a plurality of specimens of a substance to be measured by enzyme immunoassay. The process comprises:a. reacting specimens and a material which will be specifically combined with the substance to be measured and which has been made in the solid phase;b. separating the reaction mixture in step (a) into the solid phase and the liquid phase;c. reacting the solid phase with an enzyme-labelled product of a material which will be specifically combined with the substance to be measured;d. separating the reaction mixture in the step (c) into the solid phase and the liquid phase;e. reacting the solid phase or the liquid phase with a substrate for the enzyme in the step (c); andf. measuring the optical density of the reaction mixture. The improvement comprises, in step (a), successively contacting the respective specimens with the material which will be specifically combined with the substance to be measured and which has been made in the solid phase, under cooling condition at a temperature ranging from 0.degree. C. to 15.degree. C., followed by simultaneously incubating them at a temperature ranging from room temperature to 45.degree. C. The fluctuation of results can be remarkably smaller by the employment of cooling means.

摘要翻译： 用于测量物质的改进方法包括通过酶免疫测定同时测量待测物质的多个样品。 该过程包括：a。 反应样品和将与待测物质特异性结合并在固相中制备的材料; b。 将步骤（a）中的反应混合物分离成固相和液相; C。 使固相与待测物质特异性组合的材料的酶标记产物反应; d。 将步骤（c）中的反应混合物分离成固相和液相; e。 在步骤（c）中使固相或液相与酶的底物反应; 和f。 测量反应混合物的光密度。 改进包括在步骤（a）中，在冷却条件下，在0℃的温度下，将相应的试样与待测物质特异性结合的材料相继接触，并在固相中制成 至15℃，然后在室温至45℃的温度下同时孵育。通过使用冷却装置，结果的波动可以显着更小。

公开(公告)号：US4588681A

公开(公告)日：1986-05-13

申请号：US535457

申请日：1983-09-23

申请人： Takashi Sawada ,  Tomiaki Morimoto ,  Isao Miyoshi ,  Hirokuni Taguchi ,  Junichi Tohmatsu ,  Toyohiro Kitamura

发明人： Takashi Sawada ,  Tomiaki Morimoto ,  Isao Miyoshi ,  Hirokuni Taguchi ,  Junichi Tohmatsu ,  Toyohiro Kitamura

IPC分类号： G01N33/574 ,  A61K35/12 ,  A61K35/14 ,  A61K39/00 ,  A61K39/21 ,  G01N33/531 ,  G01N33/569 ,  C12Q1/70 ,  B65D71/00 ,  G01N33/53 ,  G01N33/554

CPC分类号： G01N33/531 ,  G01N33/56972 ,  G01N33/56988 ,  A61K39/00 ,  Y10S435/81 ,  Y10S435/948 ,  Y10S435/961 ,  Y10S435/975 ,  Y10S436/804 ,  Y10S436/808 ,  Y10S436/811 ,  Y10S436/813

摘要： A process for producing an adult T cell leukemia associated antigen is disclosed wherein an adult T cell leukemia associated antigen producing cell is treated with a surfactant. A method for assaying adult T cell leukemia associated antibodies by enzymoimmunoassay, radioimmunoassay or passive hemagglutination is also disclosed. In this method, the adult T cell leukemia associated antigen obtained by treating the adult T cell leukemia associated antigen producing cells with a surfactant is used as the antigen in the assay procedure.

摘要翻译： 公开了一种用于产生成人T细胞白血病相关抗原的方法，其中用表面活性剂处理成年T细胞白血病相关抗原产生细胞。 还公开了通过酶免疫测定，放射免疫测定或被动血凝测定成体T细胞白血病相关抗体的方法。 在该方法中，在测定程序中使用通过用表面活性剂处理成人T细胞白血病相关抗原产生细胞获得的成年T细胞白血病相关抗原作为抗原。

公开(公告)号：US4132767A

公开(公告)日：1979-01-02

申请号：US783460

申请日：1977-03-31

申请人： Jun-ichi Tohmatsu ,  Tomiaki Morimoto ,  Emiko Kishi

发明人： Jun-ichi Tohmatsu ,  Tomiaki Morimoto ,  Emiko Kishi

IPC分类号： C07K16/18 ,  G01N33/531 ,  A61K43/00 ,  G01N33/16

CPC分类号： C07K16/18 ,  G01N33/531 ,  Y10S436/811 ,  Y10S436/82 ,  Y10S530/806 ,  Y10S530/83

摘要： Process for the preparation of a novel .alpha.-L antibody comprising immunizing an animal other than a human with the .alpha.-L antigen or .alpha.-L antigen/.alpha.-L antibody complex, and collecting antiserum from the animal; a method for the purification of .alpha.-L antigen comprising subjecting the material containing the .alpha.-L antigen to the density gradient centrifugation, and collecting a fraction having a buoyant density less than 1.1 g/ml; a reagent comprising .alpha.-L antigen; and a reagent comprising .alpha.-L antibody for detection of the .alpha.-L antigen and the .alpha.-L antibody, such detection being useful for the diagnosis of liver diseases and examination of the blood for blood transfusion.

摘要翻译： 用于制备新的α-L抗体的方法，包括用α-L抗原或α-L抗原/α-L抗体复合物免疫除人以外的动物，以及从动物收集抗血清; 用于纯化α-L抗原的方法，包括使含有α-L抗原的物质进行密度梯度离心，并收集浮力密度小于1.1g / ml的级分; 包含α-L抗原的试剂; 和包含用于检测α-L抗原和α-L抗体的α-L抗体的试剂，所述检测可用于诊断肝脏疾病和血液检查用于输血。