公开(公告)号：US06737275B2

公开(公告)日：2004-05-18

申请号：US10068305

申请日：2002-02-05

申请人： Neil Purdie ,  Justin A. Krouse ,  Joe Studer ,  Adrian D. Marais

发明人： Neil Purdie ,  Justin A. Krouse ,  Joe Studer ,  Adrian D. Marais

IPC分类号： G01N3392

CPC分类号： G01N33/92 ,  G01N2800/044

摘要： The invention presents a method designed to simultaneously measure certain unsaturated lipids and certain vitamins present either as single substances or in complex mixtures such as exist in serum and natural oils. Target lipids are free cholesterol, unsaturated cholesteryl esters; free polyunsaturated fatty acids, and their esters as triglycerides, and phospholipids. Distributions of these analytes over the broad range of serum lipoproteins from chylomicrons to high density fractions are determined using a procedure that involves a single step reaction in which the molecular unsaturations are subjected to non-enzymatic color inducing reagents. For natural oils and vitamins, the same method serves as a quality control procedure. Analytical detection is achieved using broad spectrum absorbance and/or fluorescence measurements. Measured spectra are aggregates of the absorbance contributions from each of the analytes. Data analyses follow two paths. One uses raw spectral data. In the other, multivariate methods of analysis, particularly principal component (or factor) analysis, leads to 2-D and 3-D clustering correlations which have significant diagnostics capabilities for the early detection of human serum disorders and for quality control.

摘要翻译： 本发明提供了一种设计用于同时测量某些不饱和脂质和作为单一物质存在的某些维生素或者存在于血清和天然油中的复杂混合物中的方法。 目标脂质是游离胆固醇，不饱和胆固醇酯; 游离多不饱和脂肪酸及其作为甘油三酯的酯和磷脂。 使用涉及分子不饱和度经受非酶促诱导试剂的单步反应的程序来测定这些分析物在从乳糜微粒到高密度级分的血清脂蛋白的范围内的分布。 对于天然油和维生素，相同的方法用作质量控制程序。 使用广谱吸光度和/或荧光测量实现分析检测。 测量光谱是每种分析物的吸光度贡献的聚集。 数据分析遵循两条路径。 一个使用原始光谱数据。 另一方面，多变量分析方法，特别是主成分（或因子）分析导致二维和三维聚类相关性，其具有早期检测人类血清障碍和质量控制的显着诊断能力。