利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

5 条记录 (耗时 0.013 秒)

    Direct serum lipids assays for evaluation of disease states
    3.
    发明授权
    Direct serum lipids assays for evaluation of disease states 失效
    用于评估疾病状态的直接血清脂质测定

    公开(公告)号：US06737275B2

    公开(公告)日：2004-05-18

    申请号：US10068305

    申请日：2002-02-05

    申请人： Neil Purdie ,  Justin A. Krouse ,  Joe Studer ,  Adrian D. Marais

    发明人： Neil Purdie ,  Justin A. Krouse ,  Joe Studer ,  Adrian D. Marais

    IPC分类号： G01N3392

    CPC分类号： G01N33/92 ,  G01N2800/044

    摘要： The invention presents a method designed to simultaneously measure certain unsaturated lipids and certain vitamins present either as single substances or in complex mixtures such as exist in serum and natural oils. Target lipids are free cholesterol, unsaturated cholesteryl esters; free polyunsaturated fatty acids, and their esters as triglycerides, and phospholipids. Distributions of these analytes over the broad range of serum lipoproteins from chylomicrons to high density fractions are determined using a procedure that involves a single step reaction in which the molecular unsaturations are subjected to non-enzymatic color inducing reagents. For natural oils and vitamins, the same method serves as a quality control procedure. Analytical detection is achieved using broad spectrum absorbance and/or fluorescence measurements. Measured spectra are aggregates of the absorbance contributions from each of the analytes. Data analyses follow two paths. One uses raw spectral data. In the other, multivariate methods of analysis, particularly principal component (or factor) analysis, leads to 2-D and 3-D clustering correlations which have significant diagnostics capabilities for the early detection of human serum disorders and for quality control.

    摘要翻译： 本发明提供了一种设计用于同时测量某些不饱和脂质和作为单一物质存在的某些维生素或者存在于血清和天然油中的复杂混合物中的方法。 目标脂质是游离胆固醇，不饱和胆固醇酯; 游离多不饱和脂肪酸及其作为甘油三酯的酯和磷脂。 使用涉及分子不饱和度经受非酶促诱导试剂的单步反应的程序来测定这些分析物在从乳糜微粒到高密度级分的血清脂蛋白的范围内的分布。 对于天然油和维生素，相同的方法用作质量控制程序。 使用广谱吸光度和/或荧光测量实现分析检测。 测量光谱是每种分析物的吸光度贡献的聚集。 数据分析遵循两条路径。 一个使用原始光谱数据。 另一方面，多变量分析方法，特别是主成分（或因子）分析导致二维和三维聚类相关性，其具有早期检测人类血清障碍和质量控制的显着诊断能力。

    Circular dichroism and spectrophotometric absorption detection methods and apparatus
    4.
    发明授权
    Circular dichroism and spectrophotometric absorption detection methods and apparatus 失效
    圆二色性和分光光度吸收检测方法及仪器

    公开(公告)号：US5252488A

    公开(公告)日：1993-10-12

    申请号：US785998

    申请日：1991-10-31

    申请人： Neil Purdie

    发明人： Neil Purdie

    IPC分类号： G01N21/19 ,  G01N21/75 ,  G01N21/78 ,  G01N33/74 ,  G01N33/92 ,  G01N33/52

    CPC分类号： G01N21/19 ,  G01N33/743 ,  G01N33/92 ,  G01N21/75 ,  G01N21/78 ,  G01N2800/044

    摘要： Spectrophotometric methods, including the use of circular dichroism, spectrophotometric absorption detection fluorescence and derivative spectrophotometric absorption methods for use in clinical chemistry detection methods. More specifically, with the use of such spectrophotometric methods in the measurement of cholesterol levels and direct measurement of cholesterol subfractions in test samples. The invention is also concerned with providing certain absorption detection spectrophotometric apparatus and spectrophotometric reagents useful in the aforesaid methods.

    摘要翻译： 分光光度法，包括使用圆二色性，分光光度吸收检测荧光和衍生分光光度吸收法用于临床化学检测方法。 更具体地说，使用这种分光光度法测量胆固醇水平并直接测量测试样品中的胆固醇亚类。 本发明还涉及提供一些可用于上述方法的吸收检测分光光度计和分光光度计。

    Circular dichroism and spectrophotometric absorption detection methods
    5.
    发明授权
    Circular dichroism and spectrophotometric absorption detection methods 失效
    圆二色性和分光光度吸收检测方法

    公开(公告)号：US5246864A

    公开(公告)日：1993-09-21

    申请号：US639222

    申请日：1991-01-09

    申请人： Neil Purdie

    发明人： Neil Purdie

    IPC分类号： G01N21/19 ,  G01N21/75 ,  G01N21/78 ,  G01N33/74 ,  G01N33/92

    CPC分类号： G01N33/743 ,  G01N21/19 ,  G01N33/92 ,  G01N21/75 ,  G01N21/78 ,  G01N2800/044 ,  Y10T436/14 ,  Y10T436/141111 ,  Y10T436/142222

    摘要： Spectrophotometric methods, including the use of convention spectroscopic absorption or circular dichroism, for clinical chemistry detection methods. More specifically, with the use of such spectrophotometric methods in the measurement of cholesterol levels and direct measurement of cholesterol subfractions in clinical samples, and in the measurement of lipoprotein levels in a clinical test sample, as well as in the detection of anabolic steroids and other steroid products. The invention is also concerned with providing certain CD and conventional spectrophotometric apparatus useful in each of the aforesaid chemical methods.

    摘要翻译： 分光光度法，包括使用常规光谱吸收或圆二色性，用于临床化学检测方法。 更具体地说，通过使用这种分光光度法测定胆固醇水平并直接测量临床样品中的胆固醇亚组分，以及临床测试样品中脂蛋白水平的测量，以及合成代谢类固醇和其他 类固醇产品。 本发明还涉及提供在上述各种化学方法中有用的某些CD和常规分光光度计。