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公开(公告)号：US20140134635A1
公开(公告)日：2014-05-15
申请号：US13993930
申请日：2011-12-16
IPC分类号： G01N33/50
CPC分类号： C12N15/1079 , C12Q1/6883 , C12Q1/6886 , C12Q2600/136 , C12Q2600/178 , G01N33/5023
摘要： The invention is directed to a method of characterizing a mechanism of action of an agent (e.g., a chemotherapeutic agent, a genotoxic agent). The method comprises contacting a plurality of populations of cells with an agent to be assessed, wherein each population of cells have one gene of interest targeted by a small hairpin RNA (shRNA) and wherein said gene of interest regulates cell death and a plurality of genes that regulate cell death are targeted in the plurality of populations of cells. A responsiveness of each population of cells to the agent is determined, thereby obtaining an shRNA signature of the agent, so as to identify one or more genes that mediate a response to the agent, thereby characterizing the mechanism of action of the agent. The invention is also directed an article of manufacture for characterizing a mechanism of action of a chemotherapeutic or genotoxic agent.
摘要翻译： 本发明涉及一种表征药剂（例如，化学治疗剂，遗传毒性剂）的作用机制的方法。 该方法包括使多个细胞群与待评估的试剂接触，其中每个细胞群具有一个由小发夹RNA（shRNA）靶向的感兴趣基因，其中所述感兴趣的基因调节细胞死亡和多个基因 调节细胞死亡的目标在多个细胞群体中。 确定每种细胞群体对试剂的反应性，从而获得试剂的shRNA标签，以鉴定介导对试剂的反应的一种或多种基因，从而表征试剂的作用机制。 本发明还涉及用于表征化学治疗剂或遗传毒性剂的作用机制的制品。
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公开(公告)号：US09683231B2
公开(公告)日：2017-06-20
申请号：US13993930
申请日：2011-12-16
CPC分类号： C12N15/1079 , C12Q1/6883 , C12Q1/6886 , C12Q2600/136 , C12Q2600/178 , G01N33/5023
摘要： The invention is directed to a method of characterizing a mechanism of action of an agent (e.g., a chemotherapeutic agent, a genotoxic agent). The method comprises contacting a plurality of populations of cells with an agent to be assessed, wherein each population of cells have one gene of interest targeted by a small hairpin RNA (shRNA) and wherein said gene of interest regulates cell death and a plurality of genes that regulate cell death are targeted in the plurality of populations of cells. A responsiveness of each population of cells to the agent is determined, thereby obtaining an shRNA signature of the agent, so as to identify one or more genes that mediate a response to the agent, thereby characterizing the mechanism of action of the agent. The invention is also directed an article of manufacture for characterizing a mechanism of action of a chemotherapeutic or genotoxic agent.
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公开(公告)号：US09670486B2
公开(公告)日：2017-06-06
申请号：US13994057
申请日：2011-12-16
CPC分类号： C12N15/1079 , C12Q1/6883 , C12Q1/6886 , C12Q2600/136 , C12Q2600/178 , G01N33/5023
摘要： In one aspect, the invention is directed to a method of characterizing a mechanism of action of a combination of agents. The method comprises contacting a plurality of populations of cells with a combination of agents to be assessed, wherein each population of cells have one gene of interest targeted by a small hairpin RNA (shRNA) and wherein the gene of interest regulates cell death and a plurality of genes that regulate cell death are targeted in the plurality of populations of cells. A responsiveness of each population of cells to the combination of agents is determined, thereby obtaining an shRNA signature of the combination of agents so as to identify one or more genes that mediate a response to the combination of agents, thereby characterizing the mechanism of action of the combination of agents. In another aspect, the invention is directed to a method of determining whether a patient population treated with a first agent would benefit from a treatment using the first agent in combination with one or more additional agents. In yet another aspect, the invention is directed to method of determining whether a formulation of one or more agents maintains a mechanism of action of the one or more agents when unformulated.
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公开(公告)号：US20140206544A1
公开(公告)日：2014-07-24
申请号：US13994057
申请日：2011-12-16
CPC分类号： C12N15/1079 , C12Q1/6883 , C12Q1/6886 , C12Q2600/136 , C12Q2600/178 , G01N33/5023
摘要： In one aspect, the invention is directed to a method of characterizing a mechanism of action of a combination of agents. The method comprises contacting a plurality of populations of cells with a combination of agents to be assessed, wherein each population of cells have one gene of interest targeted by a small hairpin RNA (shRNA) and wherein the gene of interest regulates cell death and a plurality of genes that regulate cell death are targeted in the plurality of populations of cells. A responsiveness of each population of cells to the combination of agents is determined, thereby obtaining an shRNA signature of the combination of agents so as to identify one or more genes that mediate a response to the combination of agents, thereby characterizing the mechanism of action of the combination of agents. In another aspect, the invention is directed to a method of determining whether a patient population treated with a first agent would benefit from a treatment using the first agent in combination with one or more additional agents. In yet another aspect, the invention is directed to method of determining whether a formulation of one or more agents maintains a mechanism of action of the one or more agents when unformulated.
摘要翻译： 一方面，本发明涉及一种表征药剂组合的作用机理的方法。 所述方法包括使多个细胞群与待评估的试剂的组合接触，其中每个细胞群具有一个由小发夹RNA（shRNA）靶向的感兴趣基因，并且其中感兴趣的基因调节细胞死亡和多个 的调节细胞死亡的基因被靶向于多个细胞群体。 确定每种细胞群与药剂组合的反应性，从而获得试剂组合的shRNA标签，以鉴定介导对试剂组合的反应的一种或多种基因，从而表征 代理商的组合。 在另一方面，本发明涉及一种确定用第一药剂治疗的患者群体是否将受益于使用第一药剂与一种或多种另外的药剂联合治疗的方法。 在另一方面，本发明涉及确定一种或多种试剂的制剂是否在未配制时维持所述一种或多种试剂的作用机制的方法。
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