公开(公告)号：US20100143394A1

公开(公告)日：2010-06-10

申请号：US12442044

申请日：2007-09-26

申请人： Karim C. El Kasmi ,  Brad Jordan ,  Richard Kriwacki ,  Beth Mann ,  Carlos J. Orihuela ,  Elaine Tuomanen

发明人： Karim C. El Kasmi ,  Brad Jordan ,  Richard Kriwacki ,  Beth Mann ,  Carlos J. Orihuela ,  Elaine Tuomanen

IPC分类号： A61K39/02 ,  C07K14/315 ,  A61P31/04 ,  A61P37/04

CPC分类号： C07K14/3156 ,  A61K39/00

摘要： Compositions and methods for preventing and treating pneumococcal infections are provided. Compositions include novel polypeptides comprising an amino acid sequence corresponding to the R2i or R22 domain of CbpA or a consensus sequence of one of these domains, and variants and fragments thereof, wherein the polypeptide is stabilized in a desired conformation, particularly a loop conformation. The polypeptides of the invention may be engineered to comprise a first and a second cysteine residue, thereby resulting in the formation of a disulfide bond that stabilizes the polypeptide in the desired conformation. Alternatively, a polypeptide of the invention may be modified to create a synthetic linkage between a first and second amino acid residue present within the polypeptide, wherein the synthetic linkage stabilizes the polypeptide in the desired conformation. The polypeptides of the invention may further comprise an amino acid sequence for a T cell epitope. Compositions further include isolated nucleic acid molecules that encode the polypeptides of the invention, immunogenic compositions and vaccines comprising the disclosed polypeptides, and antibodies specific for these polypeptides.

摘要翻译： 提供了预防和治疗肺炎球菌感染的组合物和方法。 组合物包括包含对应于CbpA的R2i或R22结构域的氨基酸序列或这些结构域之一的共有序列及其变体和片段的新型多肽，其中所述多肽被稳定为期望的构象，特别是环构象。 本发明的多肽可被工程化以包含第一和第二半胱氨酸残基，由此导致二硫键的形成，从而将多肽稳定在所需构象。 或者，可以修饰本发明的多肽以在多肽内存在的第一和第二氨基酸残基之间产生合成连接，其中所述合成连接使所述多肽以所需构象稳定。 本发明的多肽还可以包含T细胞表位的氨基酸序列。 组合物还包括编码本发明多肽的分离的核酸分子，包含所公开的多肽的免疫原性组合物和疫苗以及对这些多肽特异的抗体。

公开(公告)号：US08722055B2

公开(公告)日：2014-05-13

申请号：US12442044

申请日：2007-09-26

申请人： Karim C. El Kasmi ,  Brad Jordan ,  Richard Kriwacki ,  Beth Mann ,  Carlos J. Orihuela ,  Elaine Tuomanen

发明人： Karim C. El Kasmi ,  Brad Jordan ,  Richard Kriwacki ,  Beth Mann ,  Carlos J. Orihuela ,  Elaine Tuomanen

IPC分类号： A61K39/02 ,  A61K39/385 ,  A61K39/085 ,  A61K39/09 ,  A61K45/00 ,  A61K47/00 ,  A61K47/48 ,  C07K16/12 ,  C07K14/005

CPC分类号： C07K14/3156 ,  A61K39/00

摘要： Compositions and methods for preventing and treating pneumococcal infections are provided. Compositions include novel polypeptides comprising an amino acid sequence corresponding to the R2i or R22 domain of CbpA or a consensus sequence of one of these domains, and variants and fragments thereof, wherein the polypeptide is stabilized in a desired conformation, particularly a loop conformation. The polypeptides of the invention may be engineered to comprise a first and a second cysteine residue, thereby resulting in the formation of a disulfide bond that stabilizes the polypeptide in the desired conformation. Alternatively, a polypeptide of the invention may be modified to create a synthetic linkage between a first and second amino acid residue present within the polypeptide, wherein the synthetic linkage stabilizes the polypeptide in the desired conformation. The polypeptides of the invention may further comprise an amino acid sequence for a T cell epitope. Compositions further include isolated nucleic acid molecules that encode the polypeptides of the invention, immunogenic compositions and vaccines comprising the disclosed polypeptides, and antibodies specific for these polypeptides.

摘要翻译： 提供了预防和治疗肺炎球菌感染的组合物和方法。 组合物包括包含对应于CbpA的R2i或R22结构域的氨基酸序列或这些结构域之一的共有序列及其变体和片段的新型多肽，其中所述多肽被稳定为期望的构象，特别是环构象。 本发明的多肽可被工程化以包含第一和第二半胱氨酸残基，由此导致二硫键的形成，从而将多肽稳定在所需构象。 或者，可以修饰本发明的多肽以在多肽内存在的第一和第二氨基酸残基之间产生合成连接，其中所述合成连接使所述多肽以所需构象稳定。 本发明的多肽还可以包含T细胞表位的氨基酸序列。 组合物还包括编码本发明多肽的分离的核酸分子，包含所公开的多肽的免疫原性组合物和疫苗以及对这些多肽特异的抗体。