公开(公告)号：US06797500B2

公开(公告)日：2004-09-28

申请号：US10273051

申请日：2002-10-17

申请人： Mario W. Chen ,  Qiong Cheng ,  Katharine Janet Gibson ,  Kristy Nan-Shan Kostichka ,  Stuart M. Thomas ,  Vasantha Nagarajan

发明人： Mario W. Chen ,  Qiong Cheng ,  Katharine Janet Gibson ,  Kristy Nan-Shan Kostichka ,  Stuart M. Thomas ,  Vasantha Nagarajan

IPC分类号： C12N902

CPC分类号： C12N9/0073 ,  C12N9/0006 ,  C12N9/0008 ,  C12N15/52 ,  C12P7/42 ,  C12P7/44 ,  C12P7/6409 ,  C12P17/08

摘要： A 10 kb gene cluster has been isolated from Rhodococcus ruber SC1 comprising genes encoding enzymes useful for the synthesis of dodecanoic diacid from cyclododecanone and other cyclic intermediates. The six specific open reading frames have been identified that are associated with dodecanoic diacid biosynthesis. In addition to the expected substrates the enzymes of the instant invention have moderate specificity for C11-C15 compounds.

摘要翻译： 已经从Rhodococcus ruber SC1中分离出10kb的基因簇，其包含编码可用于从环十二烷酮和其它环状中间体合成十二烷二酸的酶的基因。 已经鉴定了与十二烷二酸生物合成相关的六个特定的开放阅读框。 除了预期的底物外，本发明的酶对C11-C15化合物具有适度的特异性。