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公开(公告)号：US09068190B2
公开(公告)日：2015-06-30
申请号：US13539125
申请日：2012-06-29
IPC分类号： C12P21/06 , C12P7/16 , C12N9/00 , C12N1/20 , C07H21/04 , C12N15/81 , C12N9/04 , C12N9/02 , C12N9/06 , C12N9/10 , C12N9/88 , C12N15/52 , C12N15/70 , C12N15/75 , C12N15/74
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0016 , C12N9/1022 , C12N9/1096 , C12N9/88 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/746 , C12N15/75 , C12N15/81 , C12Y101/01001 , C12Y101/01002 , C12Y101/01086 , C12Y102/00 , C12Y102/01003 , C12Y102/04004 , C12Y202/01006 , C12Y401/01072 , C12Y402/01009 , Y02E50/10 , Y02E50/343 , Y02P20/52
摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
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公开(公告)号：US20120196341A1
公开(公告)日：2012-08-02
申请号：US13281872
申请日：2011-10-26
申请人： Gail K. Donaldson , Andrew C. Eliot , Vasantha Nagarajan , Charles E. Nakamura , Lixuan Lisa Huang
发明人： Gail K. Donaldson , Andrew C. Eliot , Vasantha Nagarajan , Charles E. Nakamura , Lixuan Lisa Huang
IPC分类号： C12P7/16
CPC分类号： C12N9/88 , C12N9/0006 , C12N9/1205 , C12N9/78 , C12P7/16 , C12P7/26 , Y02E50/10
摘要： Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein.
摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达2-丁醇生物合成途径的重组细菌的发酵生长来制备丁醇，优选2-丁醇。 本发明的重组微生物和方法也可适用于生产2-丁酮，本文公开的2-丁醇生物合成途径中的中间体。
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3.发明申请FERMENTIVE PRODUCTION OF ISOBUTANOL USING HIGHLY EFFECTIVE KETOL-ACID REDUCTOISOMERASE ENZYMES 审中-公开使用高效的乙醇酸还原酶制剂生产ISOBUTMOL公开(公告)号：US20110244536A1
公开(公告)日：2011-10-06
申请号：US12893077
申请日：2010-09-29
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/88 , C12N15/52 , C12P7/42 , C12Y101/01008 , C12Y101/01086 , C12Y401/01001 , C12Y401/01072 , C12Y401/02009 , C12Y402/01009 , Y02E50/10 , Y02P20/52
摘要： Ketol-acid reductoisomerase enzymes have been identified that provide high effectiveness in vivo as a step in an isobutanol biosynthetic pathway in bacteria and in yeast. These KARIs are members of a clade identified through molecular phylogenetic analysis called the SLSL Clade.
摘要翻译： 已经鉴定了酮醇酸还原异构酶，其在细菌和酵母中作为异丁醇生物合成途径的步骤在体内提供高效力。 这些KARI是通过称为SLSL分子的分子系统发育分析鉴定的进化枝的成员。
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公开(公告)号：US20110112334A1
公开(公告)日：2011-05-12
申请号：US12939315
申请日：2010-11-04
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0016 , C12N9/1022 , C12N9/1096 , C12N9/88 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/746 , C12N15/75 , C12N15/81 , C12Y101/01001 , C12Y101/01002 , C12Y101/01086 , C12Y102/00 , C12Y102/01003 , C12Y102/04004 , C12Y202/01006 , C12Y401/01072 , C12Y402/01009 , Y02E50/10 , Y02E50/343 , Y02P20/52
摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达异丁醇生物合成途径的重组细菌的发酵生长来生产丁醇，优选异丁醇。
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公开(公告)号：US20100093020A1
公开(公告)日：2010-04-15
申请号：US12634753
申请日：2009-12-10
IPC分类号： C12Q1/04
摘要： Enterococcus bacteria having enhanced tolerance to butanols have been isolated. The bacteria are useful for the fermentive production of butanol. New methods for the isolation of butanol tolerant Enterococcus are also provided.
摘要翻译： 已经分离出对丁醇具有增强耐性的肠球菌。 该细菌可用于发酵生产丁醇。 还提供了用于分离耐丁醇肠球菌的新方法。
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公开(公告)号：US20090155870A1
公开(公告)日：2009-06-18
申请号：US12111359
申请日：2008-04-29
申请人： GAIL K. DONALDSON , Andrew C. Eliot , Lori Ann Maggio-Hall , Vasantha Nagarajan , Charles E. Nakamura , Jean-Francois Tomb
发明人： GAIL K. DONALDSON , Andrew C. Eliot , Lori Ann Maggio-Hall , Vasantha Nagarajan , Charles E. Nakamura , Jean-Francois Tomb
CPC分类号： C12N15/63 , C12N9/0006 , C12N9/1205 , C12N9/78 , C12N9/88 , C12P7/16 , C12P7/26 , Y02E50/10
摘要： Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein. Specifically disclosed herein are the use of coenzyme B12-independent butanediol dehydratases that catalyzes the substrate to product conversion of 2,3-butanediol to 2-butanone in the process of producing 2-butanol and 2-butanone.
摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达2-丁醇生物合成途径的重组细菌的发酵生长来制备丁醇，优选2-丁醇。 本发明的重组微生物和方法也可适用于生产2-丁酮，本文公开的2-丁醇生物合成途径中的中间体。 本文具体公开的是在生产2-丁醇和2-丁酮的过程中催化基质以产生2,3-丁二醇转化为2-丁酮的辅酶B12非依赖性丁二醇脱水酶的用途。
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公开(公告)号：US20090068704A1
公开(公告)日：2009-03-12
申请号：US11852834
申请日：2007-09-10
申请人： Perry G. Caimi , Mario W. Chen , Vasantha Nagarajan , Jean-Francois Tomb , Siqun Wang , Yuying Zhang
发明人： Perry G. Caimi , Mario W. Chen , Vasantha Nagarajan , Jean-Francois Tomb , Siqun Wang , Yuying Zhang
IPC分类号： C12N15/31 , C12N15/52 , C07K14/00 , C12N15/63 , C12P7/20 , C12P1/04 , C12P19/04 , C12P1/02 , C12P7/00 , C12N1/21 , C12N1/15 , C12N1/19
CPC分类号： C12P7/20 , C07K2319/00 , C12N9/2451 , C12N9/246 , C12P7/18 , C12Y302/01068 , C12Y302/01133
摘要： This invention relates to a method for utilizing less purified starch in fermentation processes. One example is a recombinant E. coli containing a exogenous extracellular isoamylase activity that is capable of utilizing small oligomers containing (1,6) linkages (including but not limited to isomaltose and panose) in fermentations to produce useful products. The invention is useful in large-scale industrial biofermentations by reducing the cost of the substrate carbohydrate.
摘要翻译： 本发明涉及一种在发酵过程中利用少量纯化淀粉的方法。 一个实例是含有外源细胞外异淀粉酶活性的重组大肠杆菌，其能够利用在发酵中含有（1,6）键（包括但不限于异麦芽糖和panose）的小寡聚物以产生有用的产物。 通过降低底物碳水化合物的成本，本发明可用于大规模工业生物发酵。
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公开(公告)号：US20080182308A1
公开(公告)日：2008-07-31
申请号：US11527995
申请日：2006-09-27
申请人： Gail K. Donaldson , Lixuan Lisa Huang , Lori Ann Maggio-Hall , Vasantha Nagarajan , Charles E. Nakamura , Wonchul Suh
发明人： Gail K. Donaldson , Lixuan Lisa Huang , Lori Ann Maggio-Hall , Vasantha Nagarajan , Charles E. Nakamura , Wonchul Suh
IPC分类号： C12P7/16
CPC分类号： C12P7/16 , C12N9/0004 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/1029 , C12N9/88 , C12Y101/01157 , C12Y103/99002 , C12Y203/01009 , C12Y402/01055 , Y02E50/10 , Y02P20/52
摘要： Methods for the fermentive production of four carbon alcohols is provided. Specifically, butanol, preferably 1-butanol is produced by the fermentive growth of a recombinant bacterium expressing a 1-butanol biosynthetic pathway.
摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达1-丁醇生物合成途径的重组细菌的发酵生长来制备丁醇，优选1-丁醇。
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公开(公告)号：US07105296B2
公开(公告)日：2006-09-12
申请号：US10230026
申请日：2002-08-28
申请人： Michael G. Bramucci , Patricia C. Brzostowicz , Kristy N Kostichka , Vasantha Nagarajan , Pierre E. Rouviere , Stuart M. Thomas
发明人： Michael G. Bramucci , Patricia C. Brzostowicz , Kristy N Kostichka , Vasantha Nagarajan , Pierre E. Rouviere , Stuart M. Thomas
CPC分类号： C12N9/0073 , C12P17/08
摘要： Genes have been isolated from a variety of bacteria encoding Baeyer-Villiger monooxygenase activity. The genes and their products are useful for the conversion of ketones to the corresponding esters. A series of motifs, common to all genes, has been identified as diagnostic for genes encoding proteins of this activity.
摘要翻译： 已经从编码Baeyer-Villiger单加氧酶活性的各种细菌中分离出基因。 基因及其产物可用于将酮转化为相应的酯。 已经鉴定了所有基因共有的一系列基序作为编码该活性蛋白质的基因的诊断。
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公开(公告)号：US06797500B2
公开(公告)日：2004-09-28
申请号：US10273051
申请日：2002-10-17
申请人： Mario W. Chen , Qiong Cheng , Katharine Janet Gibson , Kristy Nan-Shan Kostichka , Stuart M. Thomas , Vasantha Nagarajan
发明人： Mario W. Chen , Qiong Cheng , Katharine Janet Gibson , Kristy Nan-Shan Kostichka , Stuart M. Thomas , Vasantha Nagarajan
IPC分类号： C12N902
CPC分类号： C12N9/0073 , C12N9/0006 , C12N9/0008 , C12N15/52 , C12P7/42 , C12P7/44 , C12P7/6409 , C12P17/08
摘要： A 10 kb gene cluster has been isolated from Rhodococcus ruber SC1 comprising genes encoding enzymes useful for the synthesis of dodecanoic diacid from cyclododecanone and other cyclic intermediates. The six specific open reading frames have been identified that are associated with dodecanoic diacid biosynthesis. In addition to the expected substrates the enzymes of the instant invention have moderate specificity for C11-C15 compounds.
摘要翻译： 已经从Rhodococcus ruber SC1中分离出10kb的基因簇，其包含编码可用于从环十二烷酮和其它环状中间体合成十二烷二酸的酶的基因。 已经鉴定了与十二烷二酸生物合成相关的六个特定的开放阅读框。 除了预期的底物外，本发明的酶对C11-C15化合物具有适度的特异性。
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